摘要：目的 设计并构建靶向甲酰肽受体FPR基因的shRNA 慢病毒表达载体并鉴定。方法 根据GenBank数据库提供的FPR基因序列，设计合成针对FPR的shRNA序列，构建PDS019_pL/shRNA/GFP/F-FPR慢病毒载体，并通过测序鉴定。结果 成功构建的重组质粒测序结果与Genebank 中的FPR cDNA 序列相符。结论 构建FPR基因shRNA干扰载体，为该基因的相关实验研究提供载体。

    关键词：FPR基因；载体构建

    Abstract：Objective To construct and identify FPR shRNA lentiviral vector. Methods Genome sequences of FPR gene was retrieved from Genebank. The shRNA sequences for FPR were synthesized and cloned into PDS019_pL/shRNA/GFP/F to generate shRNA lentiviral vector. The recombinant vectors was identified by sequencing.Results The sequence identified by sequencing were the same as the targeting one. Conclusion The constructed FPR shRNA lentiviral vectors were constructed ......