摘要：目的 对1起由诺如病毒引起的学校聚集性急性胃肠炎疫情进行实验室调查分析。方法 流行病学调查与实验室检测相结合，采集病例肛拭、粪便、食品、调味品、环境涂抹样、水样共40份样本，进行RT-PCR核酸检测及常规细菌学检测。结果 6名学生、1名保健教师、3名食堂厨师的肛拭样和1个粪便样、1个食品中检出诺如病毒核酸阳性。结论 RT-PCR检测技术能快速有效的检出肛拭、粪便、食物中的诺如病毒，从而为疫情的病因诊断提供科学依据。

    关键词：诺如病毒；PCR；食源性疾病；检测技术

    Abstract：Objective A laboratory investigation and analysis of school outbreaks of acute gastroenteritis caused by Norovirus was performed. Methods The method combined epidemiological investigation with laboratory testing was employed. A total of 40 samples including anal swabs and feces of patients； food； condiments； smear samples of environment samples ； water samples were collected and utilized for RT-PCR detection and routine bacteriological testing. Results Norovirus nucleic acid were detected positive in anal swabs of six students ......