PAs/PAI—1在膜性肾病肾组织中的动态表达及意义(1)
摘要：目的 建立膜性肾病(MN)大鼠模型，观察PAs/PAI-1在不同时间点肾组织中的动态表达规律，探讨PAs/PAI-1在MN发生过程中的意义。方法 将30只SD雄性大鼠随机分为正常组和模型组，正常组不给予任何处理，模型组按改良Border法[1]，尾静脉注射16 mg/kg C-BSA复制MN大鼠模型，于正式免疫第1、2、3、4 w取材，取材前收集24 h尿，定量动态检测24 h尿蛋白水平(24hUTP)，取大鼠肾组织进行光镜、电镜及免疫荧光鉴定成模情况，免疫组化方法动态检测肾组织中t-PA、u-PA、PAI-1的表达。结果 ①模型组大鼠肾脏病理损害随时间延长逐渐加重。②模型组第2 w起24 h尿蛋白水平显著高于正常组(P<0.05)。③模型组肾组织中t-PA、u-PA表达随时间延长呈下降趋势，PAI-1表达随时间延长呈上升趋势；t-PA表达较正常组降低，PAI-1表达较正常组升高，u-PA第1 w末出现短暂升高，以后较正常组降低，差异均有统计学意义(P<0.05)。结论 肾组织PAs/PAI-1表达失衡是导致MN病理损害、加重蛋白尿发生发展的重要因素。
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关键词：阳离子化牛血清白蛋白(c-BSA)；膜性肾病(MN)；组织型纤溶酶原激活物(t-PA)；尿激酶型纤溶酶原激活物(u-PA)；纤溶酶原激活物抑制剂-1(PAI-1)
Abstract：Objective To establish a membranous nephropathy(MN) rat model，dynamic observation of PAs/PAI-1 in the renal tissue at different time points in the expression pattern of PAs/PAI-1，in the production of MN.Methods 30 male SD rats were randomly divided into normal group and model group，the normal group was not given any treatment，model group according to the modified Border method[1]，tail vein injection of 16 mg/kg C-BSA replication model of MN rats，1，2，3，4 w in the official immunity，were collected before 24 h urine，24 h urinary protein quantitative dynamic detection level(24hUTP)，renal tissue of rats were studied by light microscopy，electron microscopy and immunofluorescence identification model.T-PA，the kidney tissue was detected with immunohistochemical method in dynamic u-PA，the expression of PAI-1.Results ①The renal pathological damage of rats in model group were aggravated with time.②The model second w 24 h urinary protein level was significantly higher than the normal group (P<0.05).③The renal tissue of t-PA model group，u The expression of-PA decreased with time，the expression of PAI-1 increased with time； the expression of t-PA decreased compared with the normal group，the expression of PAI-1 increased compared with normal group，u-PA first w appeared at the end of a transient increase，then decreased compared with the normal group，the differences were statistically significant(P<0.05).Conclusion The expression of PAs/PAI-1 is the result of imbalance the pathological damage of MN，the important factor to worsen the occurrence and development of proteinuria.
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Key words：Cationic bovine serum albumin(c-BSA)；Membranous nephropathy(MN)；Tissue type plasminogen activator(t-PA)；Urokinase type plasminogen activator (u-PA)；Plasminogen activator inhibitor-1(PAI-1)
1 資料与方法
1.1实验材料 牛血清白蛋白(购自国药集团化学试剂成都有限公司)；羊毛脂、液体石蜡、碳化二亚胺(购自厦门星隆达化学试剂公司)；PASM染色试剂盒、Masson染色试剂盒、t-PA、u-PA、PAI-1免疫组化试剂盒(购自福州迈新生物试剂有限公司)；兔抗大鼠IgG多克隆抗体及异硫氰酸荧光素标记羊抗兔荧光抗体(北京博奥森生物技术有限公司提供)。, http://www.100md.com(梁静 张渊 孟祥龙 赵玉容 王莉)
, http://www.100md.com
关键词：阳离子化牛血清白蛋白(c-BSA)；膜性肾病(MN)；组织型纤溶酶原激活物(t-PA)；尿激酶型纤溶酶原激活物(u-PA)；纤溶酶原激活物抑制剂-1(PAI-1)
Abstract：Objective To establish a membranous nephropathy(MN) rat model，dynamic observation of PAs/PAI-1 in the renal tissue at different time points in the expression pattern of PAs/PAI-1，in the production of MN.Methods 30 male SD rats were randomly divided into normal group and model group，the normal group was not given any treatment，model group according to the modified Border method[1]，tail vein injection of 16 mg/kg C-BSA replication model of MN rats，1，2，3，4 w in the official immunity，were collected before 24 h urine，24 h urinary protein quantitative dynamic detection level(24hUTP)，renal tissue of rats were studied by light microscopy，electron microscopy and immunofluorescence identification model.T-PA，the kidney tissue was detected with immunohistochemical method in dynamic u-PA，the expression of PAI-1.Results ①The renal pathological damage of rats in model group were aggravated with time.②The model second w 24 h urinary protein level was significantly higher than the normal group (P<0.05).③The renal tissue of t-PA model group，u The expression of-PA decreased with time，the expression of PAI-1 increased with time； the expression of t-PA decreased compared with the normal group，the expression of PAI-1 increased compared with normal group，u-PA first w appeared at the end of a transient increase，then decreased compared with the normal group，the differences were statistically significant(P<0.05).Conclusion The expression of PAs/PAI-1 is the result of imbalance the pathological damage of MN，the important factor to worsen the occurrence and development of proteinuria.
, http://www.100md.com
Key words：Cationic bovine serum albumin(c-BSA)；Membranous nephropathy(MN)；Tissue type plasminogen activator(t-PA)；Urokinase type plasminogen activator (u-PA)；Plasminogen activator inhibitor-1(PAI-1)
1 資料与方法
1.1实验材料 牛血清白蛋白(购自国药集团化学试剂成都有限公司)；羊毛脂、液体石蜡、碳化二亚胺(购自厦门星隆达化学试剂公司)；PASM染色试剂盒、Masson染色试剂盒、t-PA、u-PA、PAI-1免疫组化试剂盒(购自福州迈新生物试剂有限公司)；兔抗大鼠IgG多克隆抗体及异硫氰酸荧光素标记羊抗兔荧光抗体(北京博奥森生物技术有限公司提供)。, http://www.100md.com(梁静 张渊 孟祥龙 赵玉容 王莉)