牛奶和蛋白类饮料防腐剂采取SPE—HPLC法检测的效果分析(1)
摘要:目的 研究牛奶和蛋白类饮料防腐剂测定中采取固相萃取-高效液相色谱法(SPE-HPLC)的效果。方法 将牛奶和蛋白类饮料中提取的防腐剂作为研究对象,分别采用两种检测方式完成牛奶和蛋白类饮料中防腐剂的测定,对照组采用常规的TLC(薄层色谱法)进行检测,观察组则采用固相萃取-高效液相色谱法进行检测,两组均进行0.2 g样品量的称取,对这两种检测方式的检测结果与产品回收率进行对比分析。结果 对照组乳饮品、蛋白、牛奶中的纯度与分离率显著高于对照组;观察组的样品回收率为99.80%,显著高于对照组的82.00%(P<0.05)。结论 固相萃取-高效液相色谱法测定牛奶和蛋白类饮料防腐剂的效果显著,具有简单、快捷、回收率高等优点,值得广泛推广。
关键词:牛奶;蛋白类饮料;防腐剂测定;固相萃取-高效液相色谱法
中图分类号:R740 文献标识码:A 文章编号:1006-1959(2017)19-0080-03
, 百拇医药
Analysis of the Effect of SPE-HPLC Test on Preservatives in Milk and Protein Drinks
WANG Lin,XU Ling
(Chenghua District Center for Disease Control and Prevention,Chengdu 610051,Sichuan,China)
Abstract:Objective To study the effect of solid-phase extraction and high performance liquid chromatography(SPE-HPLC)on the determination of preservatives in milk and protein drinks.Methods The preservatives used in milk and protein beverage were used as the research object.The determination of preservatives in milk and protein beverage was carried out by two kinds of detection methods.The control group was tested by conventional TLC(thin layer chromatography),the observation group was treated by SPE-HPLC detection,the two groups were 0.2 g sample weighing, test results of the two kinds of detection methods and product recovery rate were analyzed.Results In the control group,milk drinks,protein purity and separation rate in milk was significantly higher than the control group;the sample recovery rate was 99.8% in the observation group,significantly higher than 82% in the control group(P<0.05).Conclusion By SPE-HPLC method for the determination of the effect of milk and protein beverage preservatives significantly,has the advantages of simple,fast,high recovery and is worthy of extensive promotion.
, 百拇医药
Key words:Milk;Protein beverage;Preservative determination;Solid phase extraction-high performance liquid chromatography
現阶段,在饮料分析过程中,固相萃取-高效液相色谱法得到了广泛应用,这种检测方式具有高效、快捷等特点[1]。随着科学技术的不断进步与发展,固相萃取-高效液相色谱法水平也在很大程度上得到提升,其分离效能、检测灵敏度、分析速度与操作自动化水平均得到改善[2]。虽然现阶段的固相萃取-高效液相色谱法得到了较大发展,但是这种检测方式依旧保留了经典液相色谱法的一些优点,例如流动相较多、样品适用范围较广、方便色谱制备等。在制药工业、食品工业、生物工程以及石油化工等诸多领域,这种检测方式均得到了广泛应用[3]。研究显示,固相萃取-高效液相色谱法在牛奶和蛋白类饮料防腐剂测定中的效果显著。本研究对此也进行了研究,总结如下。
1 仪器与试剂
1.1试剂分析
选取市场上销售的蛋白饮料、乳制饮料以及液体乳作为研究样品;羟基苯甲酸酯(TCI-GR公司提供)、脱氢乙酸(fluka公司提供)、山梨酸、甲酸(DR公司提供)、磷酸二氢钾、色谱纯、磷酸、乙腈为主要的试剂。
1.2仪器分析
ALC-110.4电子天平;美国Waters 1525型高效液相色谱仪,配备Kromasil C18柱,2996型二极管阵列检测器;XT-SPE-1固相萃取仪;PEP-SPE6cc固相萃取柱;上海科哲KH-3100薄层色谱系统;杭州奥盛KD200氮吹仪。, 百拇医药(王林 许凌)
关键词:牛奶;蛋白类饮料;防腐剂测定;固相萃取-高效液相色谱法
中图分类号:R740 文献标识码:A 文章编号:1006-1959(2017)19-0080-03
, 百拇医药
Analysis of the Effect of SPE-HPLC Test on Preservatives in Milk and Protein Drinks
WANG Lin,XU Ling
(Chenghua District Center for Disease Control and Prevention,Chengdu 610051,Sichuan,China)
Abstract:Objective To study the effect of solid-phase extraction and high performance liquid chromatography(SPE-HPLC)on the determination of preservatives in milk and protein drinks.Methods The preservatives used in milk and protein beverage were used as the research object.The determination of preservatives in milk and protein beverage was carried out by two kinds of detection methods.The control group was tested by conventional TLC(thin layer chromatography),the observation group was treated by SPE-HPLC detection,the two groups were 0.2 g sample weighing, test results of the two kinds of detection methods and product recovery rate were analyzed.Results In the control group,milk drinks,protein purity and separation rate in milk was significantly higher than the control group;the sample recovery rate was 99.8% in the observation group,significantly higher than 82% in the control group(P<0.05).Conclusion By SPE-HPLC method for the determination of the effect of milk and protein beverage preservatives significantly,has the advantages of simple,fast,high recovery and is worthy of extensive promotion.
, 百拇医药
Key words:Milk;Protein beverage;Preservative determination;Solid phase extraction-high performance liquid chromatography
現阶段,在饮料分析过程中,固相萃取-高效液相色谱法得到了广泛应用,这种检测方式具有高效、快捷等特点[1]。随着科学技术的不断进步与发展,固相萃取-高效液相色谱法水平也在很大程度上得到提升,其分离效能、检测灵敏度、分析速度与操作自动化水平均得到改善[2]。虽然现阶段的固相萃取-高效液相色谱法得到了较大发展,但是这种检测方式依旧保留了经典液相色谱法的一些优点,例如流动相较多、样品适用范围较广、方便色谱制备等。在制药工业、食品工业、生物工程以及石油化工等诸多领域,这种检测方式均得到了广泛应用[3]。研究显示,固相萃取-高效液相色谱法在牛奶和蛋白类饮料防腐剂测定中的效果显著。本研究对此也进行了研究,总结如下。
1 仪器与试剂
1.1试剂分析
选取市场上销售的蛋白饮料、乳制饮料以及液体乳作为研究样品;羟基苯甲酸酯(TCI-GR公司提供)、脱氢乙酸(fluka公司提供)、山梨酸、甲酸(DR公司提供)、磷酸二氢钾、色谱纯、磷酸、乙腈为主要的试剂。
1.2仪器分析
ALC-110.4电子天平;美国Waters 1525型高效液相色谱仪,配备Kromasil C18柱,2996型二极管阵列检测器;XT-SPE-1固相萃取仪;PEP-SPE6cc固相萃取柱;上海科哲KH-3100薄层色谱系统;杭州奥盛KD200氮吹仪。, 百拇医药(王林 许凌)
参见:首页 > 中医药 > 中医药保健 > 食疗药膳 > 食物大全 > 副食品类 > 牛奶 > 牛奶与健康