HPLC法测定不同产地附子中主要成分的含量(1)
摘 要：目的 考察不同产地的西南附子中新乌头原碱，次乌头原碱和乌头原碱的含量。方法 采用HPLC进行测定，色谱柱为Diamonsil C18 柱(150 mm×4.6 mm，5 μm)；流动相A为四氢呋喃-乙腈(15∶25)；流动相B为醋酸铵溶液(0.1 mol/L)，检测波长λ=235 nm；流速1 ml/min；柱温25 ℃。結果 不同产地的西南附子样品中生物碱类成分的含量有着明显差异，新乌头碱的含量以贵州小河的最高为0.17%，贵州兴义的最低为0.06%；次乌头碱的含量以四川江油的最高为0.28%，贵州花江的最低为0.13%；乌头碱的含量以云南腾冲与贵州水口寺的最高为0.04%，四川江油与贵州龙里的最低为0.01%。结论 西南附子中三种乌头碱的总含量以四川江油和云南腾冲产者最高。另外，HPLC测定法简单、易行，可为附子的产区扩大研究提供科学依据。
关键词：西南附子；新乌头原碱；次乌头原碱；乌头原碱；HPLC
中图分类号：R282.5 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.08.019
, 百拇医药
文章编号：1006-1959(2018)08-0062-03
Determination of Main Components in Aconite from Different Habitats by HPLC
QIN Li-fen，YANG Yu-qin
(Department of Pharmacy，Children's Hospital Affiliated to Kunming Medical University，Kunming 650228，Yunnan，China)
Abstract：Objective To investigate the content of new aconitine， sub aconitine and aconitine in Aconitum monkshood from different habitats.Methods The chromatographic column is determined by HPLC，the column is Diamonsil C18 column(150mm×4.6 mm，5μm)，the mobile phase A is tetrahydrofuran acetonitrile(15：25)，the mobile phase B is ammonium acetate solution(0.1mol/L)，the detection wavelength is λ=235 nm，the velocity is 1 ml/min，and the column temperature is 25℃.Results The contents of alkaloids in the samples from different habitats were significantly different.The content of neoaconine was 0.17% in Guizhou Xiaohe and 0.06% in Guizhou Xingyi.The content of sanaconine was Sichuan Jiangyou.The highest was 0.28%，and the lowest in Guizhou Huajiang was 0.13%；the content of aconitine was 0.04% in Yunnan Tengchong and Guizhou Shuikou Temple，and the lowest in Sichuan Jiangyou and Guizhou Longli was 0.01%.Conclusion The total content of three aconitines in Aconite in the southwest is highest in Sichuan Jiangyou and Yunnan Tengchong.In addition，the HPLC assay is simple and easy to perform，which can provide scientific basis for the expansion of aconite production areas.
, http://www.100md.com
Key words：Aconite；New aconitine；Subaconitine；Aconitine；HPLC
附子为毛莨科植物乌头的子根的加工品。具有回阳救逆、补火助阳、散寒止痛之功效，为回阳救逆之要药[1]。研究表明，附子的主要化学成分乌头碱是其有效成分[2]。有效成分含量的多少直接影响其成方制剂的临床疗效，然而不同的产地对其有效成分含量有一定影响。根据系统分析结果，除四川江油县为附子的传统产区外，在全国的适宜产区还包含四川、贵州、云南、陕西、湖南、湖北、甘肃、广西、江西、安徽等10个省区的336个县市[3]。本文以附子为对象，以新乌头原碱、次乌头原碱和乌头原碱的含量为指标进行研究，比较不同产地的西南附子中三种生物碱的含量。为西南附子质量评价奠定基础。
1 仪器与试药
1.1仪器 Aglient 1100高效液相色谱仪，包括四元泵、在线脱气机、自动进样器， 和DAD检测器，HP1100/ WIND3D英文工作站(美国安捷伦公司)；0.45 μm 微孔滤膜(天津市津腾实验设备有限公司)。AE240双量程电子分析天平，可根据需要进行调整为万分之一和十万分之档(梅特勒-托利多上海有限公司)。
1.2试药 对照品均购于中国药品生物制品检定所；附子药材均采自西南三省，经贵阳中医学院生药教研室王祥培教授鉴定为为毛茛科植物乌头的子根；甲醇和乙腈为色谱纯(德国默克公司)；水为娃哈哈纯净水；其它试剂均为分析纯。, http://www.100md.com(秦利芬 杨玉琴)
关键词：西南附子；新乌头原碱；次乌头原碱；乌头原碱；HPLC
中图分类号：R282.5 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.08.019
, 百拇医药
文章编号：1006-1959(2018)08-0062-03
Determination of Main Components in Aconite from Different Habitats by HPLC
QIN Li-fen，YANG Yu-qin
(Department of Pharmacy，Children's Hospital Affiliated to Kunming Medical University，Kunming 650228，Yunnan，China)
Abstract：Objective To investigate the content of new aconitine， sub aconitine and aconitine in Aconitum monkshood from different habitats.Methods The chromatographic column is determined by HPLC，the column is Diamonsil C18 column(150mm×4.6 mm，5μm)，the mobile phase A is tetrahydrofuran acetonitrile(15：25)，the mobile phase B is ammonium acetate solution(0.1mol/L)，the detection wavelength is λ=235 nm，the velocity is 1 ml/min，and the column temperature is 25℃.Results The contents of alkaloids in the samples from different habitats were significantly different.The content of neoaconine was 0.17% in Guizhou Xiaohe and 0.06% in Guizhou Xingyi.The content of sanaconine was Sichuan Jiangyou.The highest was 0.28%，and the lowest in Guizhou Huajiang was 0.13%；the content of aconitine was 0.04% in Yunnan Tengchong and Guizhou Shuikou Temple，and the lowest in Sichuan Jiangyou and Guizhou Longli was 0.01%.Conclusion The total content of three aconitines in Aconite in the southwest is highest in Sichuan Jiangyou and Yunnan Tengchong.In addition，the HPLC assay is simple and easy to perform，which can provide scientific basis for the expansion of aconite production areas.
, http://www.100md.com
Key words：Aconite；New aconitine；Subaconitine；Aconitine；HPLC
附子为毛莨科植物乌头的子根的加工品。具有回阳救逆、补火助阳、散寒止痛之功效，为回阳救逆之要药[1]。研究表明，附子的主要化学成分乌头碱是其有效成分[2]。有效成分含量的多少直接影响其成方制剂的临床疗效，然而不同的产地对其有效成分含量有一定影响。根据系统分析结果，除四川江油县为附子的传统产区外，在全国的适宜产区还包含四川、贵州、云南、陕西、湖南、湖北、甘肃、广西、江西、安徽等10个省区的336个县市[3]。本文以附子为对象，以新乌头原碱、次乌头原碱和乌头原碱的含量为指标进行研究，比较不同产地的西南附子中三种生物碱的含量。为西南附子质量评价奠定基础。
1 仪器与试药
1.1仪器 Aglient 1100高效液相色谱仪，包括四元泵、在线脱气机、自动进样器， 和DAD检测器，HP1100/ WIND3D英文工作站(美国安捷伦公司)；0.45 μm 微孔滤膜(天津市津腾实验设备有限公司)。AE240双量程电子分析天平，可根据需要进行调整为万分之一和十万分之档(梅特勒-托利多上海有限公司)。
1.2试药 对照品均购于中国药品生物制品检定所；附子药材均采自西南三省，经贵阳中医学院生药教研室王祥培教授鉴定为为毛茛科植物乌头的子根；甲醇和乙腈为色谱纯(德国默克公司)；水为娃哈哈纯净水；其它试剂均为分析纯。, http://www.100md.com(秦利芬 杨玉琴)