右美托咪定对大鼠脑创伤后炎症反应及认知功能的影响(1)
摘 要：目的 观察右美托咪定对大鼠脑创伤后炎症反应及认知功能的影响。方法 将20只成年雄性SD大鼠随机分为实验对照(CON)组和右美托咪定(DEX)组，各10只。应用可控皮层撞击法制备大鼠颅脑外伤模型。DEX组大鼠清醒后即可腹腔注射右美托咪定100 μg/kg，对照组不予处理，两组大鼠分别在创伤后6 h、24 h、72 h采用ELISA法对其血清TNF-α、IL-6及IL-1β水平进行检测，并进行新物体识别测试。结果 两组血清TNF-α、IL-6、IL-1β水平在脑创伤6 h、24 h、72 h比较，差异有统计学意义(P<0.05)，两组TNF-α、IL-6水平在24 h下降，并于72 h再次升高，且CON组高于DEX组，差异有统计学意义(P<0.05)；两组IL-1β水平在24 h、72 h時升高，且CON组高于DEX组，差异有统计学意义(P<0.05)；各个时间点两组大鼠新物体识别测试结果比较，差异无统计学意义(P>0.05)。结论 右美托咪定可减轻大鼠脑创伤后炎症反应，是否影响恢复期认知功能还需进一步观察。
关键词：右美托咪定；创伤；炎症反应
, http://www.100md.com
中图分类号：R614.2 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.14.044
文章编号：1006-1959(2018)14-0145-03
Abstract：Objective To observe the effects of dexmedetomidine on inflammatory reaction and cognitive function in rats after traumatic brain injury.Methods 20 adult male Sprague-Dawley rats were randomly divided into experimental control(CON)group and dexmedetomidine(DEX)group，10 each.A rat model of traumatic brain injury was prepared by controlled cortical impingement.The rats in group DEX could be intraperitoneally injected with dexmedetomidine 100μg/kg，and the control group was not treated.The two groups of rats were detected the serum levels of TNF-α，IL-6 and IL-1β in the serum of 6 h，24 h and 72 h after the trauma，and the new object recognition test was carried out.Results The levels of serum TNF-α，IL-6 and IL-1β in the two groups were compared at 6 h，24 h and 72 h after brain injury，the difference was statistically significant(P<0.05).The levels of TNF-α and IL-6 in the two groups decreased at 24 h and increased again at 72 h，and the CON group was higher than the DEX group，the difference was statistically significant(P<0.05)；the IL-1β level in the two groups was 24 h and 72 h were elevated，and the CON group was higher than the DEX group，the difference was statistically significant(P<0.05).There was no significant difference in the new object recognition test results between the two groups at each time point(P>0.05).Conclusion Dexmedetomidine can alleviate the post-traumatic inflammatory reaction in rats，and it is necessary to further observe whether it affects the cognitive function during recovery.
, 百拇医药
Key words：Dexmedetomidine；Trauma；Inflammatory response
目前大多研究关注于中重度颅脑损伤的发病及治疗[1]，对轻度脑创伤的病理生理变化及行为学变化未予以重视。右美托咪定是一种新型的α2受体激动剂，对脑损伤具有一定神经保护作用，可减少认知功能损害，但具体作用机制目前尚不明确。研究显示[2]，炎症反应是导致脑创伤后继发性脑损伤的重要原因，本实验观察右美托咪定对大鼠脑创伤后急性期炎症反应及认知功能的影响，为临床治疗提供实验依据。
1材料及方法
1.1动物模型制备及分组 本研究经天津市环湖医院实验动物伦理委员会批准，选取成年健康清洁级雄性SD大鼠20只，体重230～260 g，采用随机数表法分为实验对照组(CON)和右美托咪定组(DEX)，每组10只。模型的制备：根据文献[3]采用可控皮层撞击法制作大鼠颅脑外伤模型，采用1%戊巴比妥(50 mg/kg)对大鼠腹腔进行麻醉注射，并取俯卧位固定。消毒后将颅顶皮肤切开，并于大鼠于前囟与人字缝连线中点左旁开3.5 mm位置钻一小孔，直径约6.0 mm，不打开硬膜，根据自动控制撞击程序，将撞针经骨窗撞击大鼠左侧脑皮层顶叶，撞击速度3.5 mm/s，下陷深度在2 mm左右，留针时间400 ms，完成撞击后对伤口进行止血处理，用骨蜡封闭颅骨，将皮肤缝合。纳入标准：受伤后30 min内苏醒，活动自如。排除标准：苏醒时间>30 min，活动受限或死亡。, http://www.100md.com(郑玉珍 刘海根 陈君)
关键词：右美托咪定；创伤；炎症反应
, http://www.100md.com
中图分类号：R614.2 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.14.044
文章编号：1006-1959(2018)14-0145-03
Abstract：Objective To observe the effects of dexmedetomidine on inflammatory reaction and cognitive function in rats after traumatic brain injury.Methods 20 adult male Sprague-Dawley rats were randomly divided into experimental control(CON)group and dexmedetomidine(DEX)group，10 each.A rat model of traumatic brain injury was prepared by controlled cortical impingement.The rats in group DEX could be intraperitoneally injected with dexmedetomidine 100μg/kg，and the control group was not treated.The two groups of rats were detected the serum levels of TNF-α，IL-6 and IL-1β in the serum of 6 h，24 h and 72 h after the trauma，and the new object recognition test was carried out.Results The levels of serum TNF-α，IL-6 and IL-1β in the two groups were compared at 6 h，24 h and 72 h after brain injury，the difference was statistically significant(P<0.05).The levels of TNF-α and IL-6 in the two groups decreased at 24 h and increased again at 72 h，and the CON group was higher than the DEX group，the difference was statistically significant(P<0.05)；the IL-1β level in the two groups was 24 h and 72 h were elevated，and the CON group was higher than the DEX group，the difference was statistically significant(P<0.05).There was no significant difference in the new object recognition test results between the two groups at each time point(P>0.05).Conclusion Dexmedetomidine can alleviate the post-traumatic inflammatory reaction in rats，and it is necessary to further observe whether it affects the cognitive function during recovery.
, 百拇医药
Key words：Dexmedetomidine；Trauma；Inflammatory response
目前大多研究关注于中重度颅脑损伤的发病及治疗[1]，对轻度脑创伤的病理生理变化及行为学变化未予以重视。右美托咪定是一种新型的α2受体激动剂，对脑损伤具有一定神经保护作用，可减少认知功能损害，但具体作用机制目前尚不明确。研究显示[2]，炎症反应是导致脑创伤后继发性脑损伤的重要原因，本实验观察右美托咪定对大鼠脑创伤后急性期炎症反应及认知功能的影响，为临床治疗提供实验依据。
1材料及方法
1.1动物模型制备及分组 本研究经天津市环湖医院实验动物伦理委员会批准，选取成年健康清洁级雄性SD大鼠20只，体重230～260 g，采用随机数表法分为实验对照组(CON)和右美托咪定组(DEX)，每组10只。模型的制备：根据文献[3]采用可控皮层撞击法制作大鼠颅脑外伤模型，采用1%戊巴比妥(50 mg/kg)对大鼠腹腔进行麻醉注射，并取俯卧位固定。消毒后将颅顶皮肤切开，并于大鼠于前囟与人字缝连线中点左旁开3.5 mm位置钻一小孔，直径约6.0 mm，不打开硬膜，根据自动控制撞击程序，将撞针经骨窗撞击大鼠左侧脑皮层顶叶，撞击速度3.5 mm/s，下陷深度在2 mm左右，留针时间400 ms，完成撞击后对伤口进行止血处理，用骨蜡封闭颅骨，将皮肤缝合。纳入标准：受伤后30 min内苏醒，活动自如。排除标准：苏醒时间>30 min，活动受限或死亡。, http://www.100md.com(郑玉珍 刘海根 陈君)