miR-187在宫颈癌中的表达与临床病理关系的研究(1)
摘 要：目的 探讨miR-187在宫颈癌中的表达及与临床病理特征的关系。方法 收集78例宫颈癌组织标本，同时收取同期手术的60例正常宫颈组织标本，采用实时荧光定量PCR检测方法检测两种组织中miR-187的表达量并进行比较，同时分析癌组织中miR-187的表达水平与临床病理特征的关系。结果 宫颈癌组织中miR-187的表达量低于正常宫颈组织标本，统计学意义显著(P<0.001);宫颈癌组织中miR-187的表达水平与宫颈癌组织的分化程度、患者的临床分期及有无淋巴结转移情况有关(P<0.05)，而与患者的年龄、肿块类型、肿瘤大小及组织学类型无关(P>0.05)。结论 miR-187在宫颈癌组织中低表达，可能作为其诊断与治疗的重要靶点。
关键词：miR-187;宫颈癌;实时荧光定量PCR
中图分类号：R365 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.16.019
, 百拇医药
文章编号：1006-1959(2018)16-0069-03
Expression of miR-187 in Cervical Cancer and Its Relationship with Clinicopathological Features
WU Xiao-ling，ZHOU Gui-hua
(Department of Laboratory Medicine，Hunan Maternal and Child Health Care Hospital，Changsha 410008，Hunan，China)
Abstract：Objective To investigate the expression of miR-187 in cervical cancer and its relationship with clinicopathological features.Methods 78 cases of cervical cancer tissues were collected，60 normal cervical tissue specimens were collected at the same time from simultaneous operation.The expression of miR-187 in two kinds of tissues was detected and compared by real-time fluorescence quantitative PCR，and the relationship between the expression of miR-187 and clinicopathological features was analyzed. Results The expression of miR-187 in cervical cancer was significantly lower than that in normal cervical tissue，with statistical significance(P<0.001).The expression of miR-187 in cervical cancer was related to the degree of differentiation，clinical stage and lymph node metastasis (P<0.05)，but not to age，tumor type，tumor size and histological type(P>0.05).Conclusion Low expression of miR-187 may be an important target for diagnosis and treatment of cervical cancer.
, 百拇医药
Key words：miR-187;Cervical cancer;Real-time fluorescent quantitative PCR
宫颈癌(cervical cancer)是妇科肿瘤中最常见的恶性肿瘤之一，已成为世界各地妇女面临的具有挑战性的健康问题[1]。虽然高危型HPV感染是宫颈癌及其癌前病变发生的主要原因，然而并非所有感染高危型HPV的女性均会发生宫颈癌，这表明还有其他因素导致宫颈癌的發生与发展，因此，对宫颈癌的危险因素进行及时有效的检测十分必要[2-4]。微小RNA(miRNA/miR)是一类长度约为19～25个核苷酸的小的非编码和内源调节RNA分子，其可以结合特定基因的3'非翻译区(3'-UTR)以抑制mRNA翻译，然后损害蛋白质过程，对基因表达进行转录后的调节[5]。近年来，很多研究者发现miR-RNA在肿瘤性疾病的发展中扮演着重要角色，比如一些miR-RNA的表达能够抑制乳腺癌的发生发展等[6]。大量研究发现，上调一些致癌miR-RNA或者下调一些抑癌miR-RNA能够通过调控肿瘤细胞的细胞周期、血管生成、侵袭和转移等来促进肿瘤的发展[7，8]。miR-187作为miRNA超家族中的一种，已经表明能够抑制前列腺癌、卵巢癌、肝细胞癌等肿瘤的发展。本文检测了miR-187在宫颈癌组织与正常宫颈组织的表达及分析了miR-187的表达与临床病理特征的关系，旨在了解miR-187和宫颈癌的关系，为宫颈癌的诊治提供潜在靶点。, http://www.100md.com(吴晓玲 周桂华)
关键词：miR-187;宫颈癌;实时荧光定量PCR
中图分类号：R365 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.16.019
, 百拇医药
文章编号：1006-1959(2018)16-0069-03
Expression of miR-187 in Cervical Cancer and Its Relationship with Clinicopathological Features
WU Xiao-ling，ZHOU Gui-hua
(Department of Laboratory Medicine，Hunan Maternal and Child Health Care Hospital，Changsha 410008，Hunan，China)
Abstract：Objective To investigate the expression of miR-187 in cervical cancer and its relationship with clinicopathological features.Methods 78 cases of cervical cancer tissues were collected，60 normal cervical tissue specimens were collected at the same time from simultaneous operation.The expression of miR-187 in two kinds of tissues was detected and compared by real-time fluorescence quantitative PCR，and the relationship between the expression of miR-187 and clinicopathological features was analyzed. Results The expression of miR-187 in cervical cancer was significantly lower than that in normal cervical tissue，with statistical significance(P<0.001).The expression of miR-187 in cervical cancer was related to the degree of differentiation，clinical stage and lymph node metastasis (P<0.05)，but not to age，tumor type，tumor size and histological type(P>0.05).Conclusion Low expression of miR-187 may be an important target for diagnosis and treatment of cervical cancer.
, 百拇医药
Key words：miR-187;Cervical cancer;Real-time fluorescent quantitative PCR
宫颈癌(cervical cancer)是妇科肿瘤中最常见的恶性肿瘤之一，已成为世界各地妇女面临的具有挑战性的健康问题[1]。虽然高危型HPV感染是宫颈癌及其癌前病变发生的主要原因，然而并非所有感染高危型HPV的女性均会发生宫颈癌，这表明还有其他因素导致宫颈癌的發生与发展，因此，对宫颈癌的危险因素进行及时有效的检测十分必要[2-4]。微小RNA(miRNA/miR)是一类长度约为19～25个核苷酸的小的非编码和内源调节RNA分子，其可以结合特定基因的3'非翻译区(3'-UTR)以抑制mRNA翻译，然后损害蛋白质过程，对基因表达进行转录后的调节[5]。近年来，很多研究者发现miR-RNA在肿瘤性疾病的发展中扮演着重要角色，比如一些miR-RNA的表达能够抑制乳腺癌的发生发展等[6]。大量研究发现，上调一些致癌miR-RNA或者下调一些抑癌miR-RNA能够通过调控肿瘤细胞的细胞周期、血管生成、侵袭和转移等来促进肿瘤的发展[7，8]。miR-187作为miRNA超家族中的一种，已经表明能够抑制前列腺癌、卵巢癌、肝细胞癌等肿瘤的发展。本文检测了miR-187在宫颈癌组织与正常宫颈组织的表达及分析了miR-187的表达与临床病理特征的关系，旨在了解miR-187和宫颈癌的关系，为宫颈癌的诊治提供潜在靶点。, http://www.100md.com(吴晓玲 周桂华)