海洋中药蛎昆一号制剂对5-Fu化疗后所致动物骨髓抑制的功效研究(1)
摘 要：目的 研究蠣昆一号制剂对5-氟尿嘧啶(5-Fu)致小鼠骨髓抑制外周血中白细胞、血小板、淋巴细胞、单核细胞计数的影响。方法 纯系雄性BALB/c种小鼠60只，随机分为对照组、5-Fu、5-Fu联用重组人粒细胞集落刺激因子(rhG-CSF)、蛎昆一号制剂低、中、高剂量6个组。经腹腔注射肿瘤化疗药5-Fu建立小鼠骨髓抑制模型，对照组注射等量生理盐水;注射后第1天上午使用不同剂量的蛎昆一号制剂灌胃，对照组等量生理盐水灌胃;给药7 d后上午取眼球血进行血常规分析。结果 小鼠在注射5-Fu造模后，外周血白细胞、血小板、淋巴细胞与对照组相比均有明显降低(P<0.05)，单核细胞比率与对照组相比明显升高(P<0.05);不同剂量的蛎昆一号制剂及阳性对照(rhG-CSF)均有效地升高小鼠外周血白细胞、血小板、淋巴细胞数值，且单核细胞比率也较模型组明显降低，但以蛎昆一号制剂中剂量组的效果最为明显，复方制剂高剂量组次之。结论 蛎昆一号制剂能够明显改善5-Fu化疗药物所引起的小鼠骨髓抑制现象，增加骨髓抑制小鼠外周血白细胞、血小板、淋巴细胞数目;降低外周血中单核细胞比率，表明蛎昆一号制剂能够有效的恢复骨髓抑制小鼠造血系统，提高造血活性。
, 百拇医药
关键词：蛎昆一号制剂;5-氟尿嘧啶;骨髓抑制;血常规
中图分类号：R285.5 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.21.015
文章编号：1006-1959(2018)21-0051-05
Efficacy of Marine Chinese Medicine Likun No.1 Preparation on Animal Bone Marrow Suppression Induced by 5-Fu Chemotherapy
JIN Yan-ling1，LIU Guo-yan2，MO Shan-lie3，YE Lan4，ZHANG Yu-hai5
, 百拇医药
(1.School of Basic Medical Sciences，Guizhou Medical University，Guiyang 550025，Guizhou，China;
2.School of Pharmacy，Xiamen University/Zhongshan Hospital，Xiamen University，Xiamen 361101， Fujian，China;
3.Institute of Pharmacy，Xiamen University-Huayin Science and Technology Cancer Prevention and Rehabilitation Center，Xiamen 361101，Fujian，China，;
4.College of Basic Medicine，Guizhou Medical University，Guiyang 550025，Guizhou，China;
, 百拇医药
5.Zhongshan Hospital，Xiamen University，Xiamen 361101，Fujian，China)
Abstract： Objective To study the effects of Likun No. 1 preparation on the counts of leukocytes， platelets， lymphocytes and monocytes in peripheral blood of mice induced by 5-fluorouracil (5-Fu). Methods 60 male BALB/c mice were randomly divided into control group， 5-Fu， 5-Fu combined with recombinant human granulocyte colony-stimulating factor (rhG-CSF)，Likun No. 1 preparation has 6 groups of low， medium and high doses. The mouse bone marrow suppression model was established by intraperitoneal injection of the tumor chemotherapeutic drug 5-Fu. The control group was injected with the same amount of normal saline. On the first day after the injection， different doses of the Likun No. 1 preparation were administered by the stomach， and the control group was given the same amount of normal saline. After 7 d of administration， eyeballs were taken for routine blood analysis. Results After injection of 5-Fu， the peripheral blood leukocytes， platelets and lymphocytes were significantly lower than the control group (P<0.05)， and the monocyte ratio was significantly higher than that of the control group (P<0.05). Different doses of Likun No. 1 preparation and positive control (rhG-CSF) effectively increased the peripheral blood leukocyte， platelet and lymphocyte values in mice， and the monocyte ratio was also significantly lower than that of the model group. The effect of the medium dose group of Likun No.1 preparation was the most obvious， followed by the high dose group of the compound preparation. Conclusion Likun No.1 preparation can significantly improve the bone marrow suppression induced by 5-Fu chemotherapy drugs， increase the number of peripheral blood leukocytes， platelets and lymphocytes in mice， and decrease the percentage of monocytes in peripheral blood. Likun No. 1 preparation can effectively restore the hematopoietic system of myelosuppressive mice and increase hematopoietic activity., 百拇医药(靳艳玲 刘国彦 莫善列 叶 兰 张宇海)
, 百拇医药
关键词：蛎昆一号制剂;5-氟尿嘧啶;骨髓抑制;血常规
中图分类号：R285.5 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.21.015
文章编号：1006-1959(2018)21-0051-05
Efficacy of Marine Chinese Medicine Likun No.1 Preparation on Animal Bone Marrow Suppression Induced by 5-Fu Chemotherapy
JIN Yan-ling1，LIU Guo-yan2，MO Shan-lie3，YE Lan4，ZHANG Yu-hai5
, 百拇医药
(1.School of Basic Medical Sciences，Guizhou Medical University，Guiyang 550025，Guizhou，China;
2.School of Pharmacy，Xiamen University/Zhongshan Hospital，Xiamen University，Xiamen 361101， Fujian，China;
3.Institute of Pharmacy，Xiamen University-Huayin Science and Technology Cancer Prevention and Rehabilitation Center，Xiamen 361101，Fujian，China，;
4.College of Basic Medicine，Guizhou Medical University，Guiyang 550025，Guizhou，China;
, 百拇医药
5.Zhongshan Hospital，Xiamen University，Xiamen 361101，Fujian，China)
Abstract： Objective To study the effects of Likun No. 1 preparation on the counts of leukocytes， platelets， lymphocytes and monocytes in peripheral blood of mice induced by 5-fluorouracil (5-Fu). Methods 60 male BALB/c mice were randomly divided into control group， 5-Fu， 5-Fu combined with recombinant human granulocyte colony-stimulating factor (rhG-CSF)，Likun No. 1 preparation has 6 groups of low， medium and high doses. The mouse bone marrow suppression model was established by intraperitoneal injection of the tumor chemotherapeutic drug 5-Fu. The control group was injected with the same amount of normal saline. On the first day after the injection， different doses of the Likun No. 1 preparation were administered by the stomach， and the control group was given the same amount of normal saline. After 7 d of administration， eyeballs were taken for routine blood analysis. Results After injection of 5-Fu， the peripheral blood leukocytes， platelets and lymphocytes were significantly lower than the control group (P<0.05)， and the monocyte ratio was significantly higher than that of the control group (P<0.05). Different doses of Likun No. 1 preparation and positive control (rhG-CSF) effectively increased the peripheral blood leukocyte， platelet and lymphocyte values in mice， and the monocyte ratio was also significantly lower than that of the model group. The effect of the medium dose group of Likun No.1 preparation was the most obvious， followed by the high dose group of the compound preparation. Conclusion Likun No.1 preparation can significantly improve the bone marrow suppression induced by 5-Fu chemotherapy drugs， increase the number of peripheral blood leukocytes， platelets and lymphocytes in mice， and decrease the percentage of monocytes in peripheral blood. Likun No. 1 preparation can effectively restore the hematopoietic system of myelosuppressive mice and increase hematopoietic activity., 百拇医药(靳艳玲 刘国彦 莫善列 叶 兰 张宇海)