高效氯氰菊酯暴露对小鼠子宫内膜蜕膜化的影响(1)
摘要：目的 探討高效氯氰菊酯暴露对小鼠子宫内膜蜕膜化的影响。方法 将SPF级断奶昆明雌鼠40只随机分为对照组和实验组，每组20只，以灌胃方式染毒90 d。对照组给予玉米油灌胃;实验组给予10 mg/(kg·bw·d)β-CP+玉米油灌胃。染毒结束后，随机抽取对照组和实验组各10只发情期雌鼠分别与雄鼠合拢，次日清晨见阴栓记为孕1 d(D1)，于D8 d处死小鼠。各组剩余雌鼠与输精管结扎的雄鼠合笼建立假孕模型，于假孕第4天(PD4)小鼠宫角注射玉米油。D8和PD8 d摘取小鼠子宫，观察胚胎着床情况和蜕膜化诱导情况及镜下观察子宫内膜厚度。采用RT-PCR和ELISA法分别检测检测蜕膜化标志分子dtPRP和BMP2的表达。结果 β-CP暴露降低了小鼠蜕膜化诱导成功率，降低了子宫内膜厚度及dtPRP和BMP2的表达(P<0.05)。与对照组的人工诱导蜕膜化成功率(100.00%)相比，实验组小鼠的人工诱导蜕膜化成功率(30%)下降，差异有统计学意义(？字2=10.77，P<0.05)。结论 β-CP暴露通过抑制dtPRP和BMP2的表达影响小鼠子宫内膜的蜕膜化，降低了子宫内膜厚度，影响了胚胎着床。
, 百拇医药
关键词：高效氯氰菊酯;子宫内膜蜕膜化;小鼠
中图分类号：R114;R711.6; 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.03.020
文章编号：1006-1959(2019)03-0061-04
Abstract：Objective To study the effect of beta-cypermethrin on deciduation of endometrium in female mice. Methods 40 female Kunming mice of SPF grade weaning were randomly divided into two groups： control group and contamintated group with 20 each，and were contamintated by the way of lavage for 90 d.The control group was lavaged with corn oil.The contamintated group was lavaged with 10mg/(kg·bw·d)β-CP+corn oil.At the end of contamination， 10 female mice in the control group and 10 mice in the contamintated group were randomly selected to put in a same cage with the male mouse respectively. Early the next morning，Yin suppository was recorded as the first day of pregnancy(D1)，and the mice were killed on D8 d.The model of pseudopregnancy was established by putting the remaining female mice in each group and male mice ligated by vasectomy in a same cage.Corn oil was injected into the uterine horn of mice on the 4th day of pseudopregnition(PD4).The embryo implantation， decidualization induction and endometrial thickness were observed by D8 and PD8 d.The expression of dtPRP and BMP2 were detected by RT-PCR and ELISA，respectively.Results β-CP contamination decreased the success rate of decidualization induction， the thickness of endometrium and the expression of dtPRP and BMP2 in mouse decidua (P<0.05).Compared with the control group， the success rate of artificial induced deciduation (100.00%) in the experimental group decreased (30%)， and the difference was statistically significant (？字2=10.77， P<0.05).Conclusion β-CP contamination affects decidualization of mice endometrium by inhibiting the expression of dtPRP and BMP2，decreases the thickness of endometrium and affects embryo implantation., 百拇医药(周永江 方方 李超 高明妃 郭泽莉)
, 百拇医药
关键词：高效氯氰菊酯;子宫内膜蜕膜化;小鼠
中图分类号：R114;R711.6; 文献标识码：A DOI：10.3969/j.issn.1006-1959.2019.03.020
文章编号：1006-1959(2019)03-0061-04
Abstract：Objective To study the effect of beta-cypermethrin on deciduation of endometrium in female mice. Methods 40 female Kunming mice of SPF grade weaning were randomly divided into two groups： control group and contamintated group with 20 each，and were contamintated by the way of lavage for 90 d.The control group was lavaged with corn oil.The contamintated group was lavaged with 10mg/(kg·bw·d)β-CP+corn oil.At the end of contamination， 10 female mice in the control group and 10 mice in the contamintated group were randomly selected to put in a same cage with the male mouse respectively. Early the next morning，Yin suppository was recorded as the first day of pregnancy(D1)，and the mice were killed on D8 d.The model of pseudopregnancy was established by putting the remaining female mice in each group and male mice ligated by vasectomy in a same cage.Corn oil was injected into the uterine horn of mice on the 4th day of pseudopregnition(PD4).The embryo implantation， decidualization induction and endometrial thickness were observed by D8 and PD8 d.The expression of dtPRP and BMP2 were detected by RT-PCR and ELISA，respectively.Results β-CP contamination decreased the success rate of decidualization induction， the thickness of endometrium and the expression of dtPRP and BMP2 in mouse decidua (P<0.05).Compared with the control group， the success rate of artificial induced deciduation (100.00%) in the experimental group decreased (30%)， and the difference was statistically significant (？字2=10.77， P<0.05).Conclusion β-CP contamination affects decidualization of mice endometrium by inhibiting the expression of dtPRP and BMP2，decreases the thickness of endometrium and affects embryo implantation., 百拇医药(周永江 方方 李超 高明妃 郭泽莉)