摘要：目的" 探索一种稳定、经济、实用的SD新生鼠海马神经元的原代培养方法。方法" 选用出生24 h内的SD新生鼠，分离新生大鼠双侧海马组织制备海马组织单细胞悬液，采用DMEM/F12+2%B27培养体系维持培养，观察海马神经元形态变化，取培养5 d的海马神经元原代培养细胞，采用微管相关蛋白2(MAP2)免疫荧光染色鉴定神经元纯度。结果" 提取的海马神经元细胞存活率高，生长状态良好；MAP2免疫荧光鉴定神经元纯度达(95.20±1.50)%。结论" 本培养方法在保证海马神经元细胞纯度及细胞活性的同时，降低原代神经元培养成本，是一种稳定、经济、实用的海马神经元原代培养方法。

    关键词：海马；神经元；原代；培养；微管相关蛋白2

    中图分类号：R741" " " " " " " " " " " " " " " " " 文献标识码：A" " " " " " " " " " " " " " " " " DOI：10.3969/j.issn.1006-1959.2023.17.021

    文章编号：1006-1959(2023)17-0111-04

    Improvement and Identification of Primary Cell Culture Method of Neonatal Rat Hippocampal Neurons

    YUE Yu-jiao XU Ping

    (Department of Neuro-rehabilitation1，Department of Neurological2，Affiliated Hospital of Zunyi Medical University，

    Zunyi 563000，Guizhou，China)

    Abstract：Objective" To explore a stable， economical and practical primary culture method of hippocampal neurons in SD neonatal rats.Methods" SD neonatal rats within 24 hours after birth were selected. The bilateral hippocampal tissues of neonatal rats were isolated to prepare single cell suspension of hippocampal tissues. The DMEM/F12+2%B27 culture system was used to maintain culture. The morphological changes of hippocampal neurons were observed. The primary cultured cells of hippocampal neurons cultured for 5 days were taken ......