摘要：肝胆疾病种类繁多，其中有不少常规治疗效果有限的危重疾病，尤其是涉及代谢、消耗等问题的疾病需要新的治疗手段。CRISPR/Cas9是目前常见的基因编辑技术之一，具有原理简单，操作方便灵活等优点，可满足肝胆疾病的基因研究需求。近年来，已有不少使用CRISPR/Cas9研究治疗肝胆疾病的成果出现，甚至不乏疑难杂症的相关研究。本综述通过回顾近10年相关研究，从多个肝胆外科疾病角度逐一叙述并分析基因编辑技术的优势与潜力，为将来实现基因编辑技术大规模应用于临床提供参考。

    关键词：基因编辑;肝病;胆道疾病;肝移植

    中图分类号：R575" " " " " " " " " " " " " " " " " "文献标识码：A" " " " " " " " " " " " " " " " "DOI：10.3969/j.issn.1006-1959.2025.01.035

    文章编号：1006-1959(2025)01-0168-07

    Application of Gene Editing in Hepatobiliary Surgery

    WANG Xiao1， SHEN Yinfeng2，3

    (1.The First Clinical Medical College， Hubei University of Chinese Medicine， Wuhan 430065， Hubei， China;

    2.Department of Hepatobiliary Surgery， Hubei Provincial Hospital of Traditional Chinese Medicine， Affiliated

    with Hubei University of Traditional Chinese Medicine， Wuhan 430061， Hubei， China;

    3.Hubei Shizhen Laboratory， Wuhan 430065， Hubei， China)

    Abstract： There are various types of hepatobiliary diseases， among which many critical diseases getting limited therapeutic effects after routine treatment need new treatment methods， especially for those involving metabolism ......