摘要：目的" 探究荧光定量反转录聚合酶链反应(RT-PCR)对GⅠ型、GⅡ型诺如病毒(NV)的检测效能。方法" 以2020年12月-2023年12月南昌市湾里管理局疾病预防控制中心收治的50例疑似NV感染患者为研究对象，采集其粪便样本，依次应用常规RT-PCR与荧光定量RT-PCR检测方式进行检验，比较两种检验方式的阳性检出率及检测效能(检测准确性、敏感度、特异度)。结果" 荧光定量RT-PCR对GⅠ型、GⅡ型诺如病毒的阳性检出率高于常规RT-PCR检测(P<0.05)。荧光定量RT-PCR对GⅠ型、GⅡ型诺如病毒的检测准确性、敏感度、特异度高于常规RT-PCR检测(P<0.05)。结论" 荧光定量RT-PCR在GⅠ型、GⅡ型诺如病毒诊断中具有较高检测效能。

    关键词：诺如病毒；荧光定量反转录聚合酶链反应；GⅠ型；GⅡ型；检测效能

    中图分类号：R445.1" " " " " " " " " " " " " " " " " " " "文献标识码：A" " " " " " " " " " " " " "DOI：10.3969/j.issn.1006-1959.2025.06.022

    文章编号：1006-1959(2025)06-0127-04

    Detection Efficiency of Fluorescence Quantitative Reverse Transcription

    Polymerase Chain Reaction for GⅠ and GⅡ Norovirus

    XIAO Chaoguang

    (Inspection Unit， Nanchang Wanli Administration Center for Disease Control and Prevention， Nanchang 330004， Jiangxi， China)

    Abstract： Objective" To explore the detection efficiency of fluorescence quantitative reverse transcription polymerase chain reaction (RT-PCR) for GⅠ and GⅡ norovirus (NV). Methods" From December 2020 to December 2023 ......