Studyon theRoleofPELP1in AngiogenesisofEpithelial Ovarian Cancer

    XIELelel，WUJiangling2，ZHANGWenwen3，HUANGXiyue]，YANGXiao1，WANGJia1 (DepartmentofObstetricsndGyecology，DepartmentofClincalLaboratory，DepartmentofPathology，theUnversityTown Hospital of ChongqingMedical University，Chongqing ，China)

    Astract：Ueeesteeoleprouotegeso MethodsNoalovarianepithelialellISE8OndepithelialovarancancercellsOVCAR-3ndHeyereselectedHeyCtrlgroupassetas blankcontroloioldeaie PELP1wasdetedyeal-teuoresceceuanitativePCR(RTCR)ndWestBlot(WB)TerelativeexpressnevelofPwas detectedbyWB.TheconcentrationofVEGFAinthesupernatant(CM)ofOE-Vector-CMgroupandOE-PELP1-CMgroupwasdeterminedby enzyme-linkedimmunosorbents(ELA)efectsofCMontemigraioandubefoationofuanbilcalveinedothelialels (HUVECs)weredetectedbyTranswellandangiogeesisethodsResultseexpresionlevelofELPinepithelialovariancanceels OVCAR-3 and Heywas higher than that in normal ovarian epithelial cells IOSE80( Plt;0 . 0 5 ) .The expressionlevel of PELP1 in Hey-OE-PELP1 group was higher than that in Hey-Ctrl group and Hey-OE-Vector group .The concentration of VEGFA ......