费炳友

    【摘 要】目的：分析探究FISH技术在乳腺癌HER-2基因检测中的应用价值。方法：在2018年8月至2021年8月期间选择42例乳腺癌患者展开回顾性分析，以上42例患者采集的标本均接受FISH技术和IHC技术对其HER-2基因进行相应的检测，并比较不同检测技术的阳性符合率，观察经FISH技术对17号染色体多体发生的检测情况。结果：经不同检测技术检测其结果一致性相对较高(Kappa=0.544，P0.05)。结论：在乳腺癌HER-2基因检测中无论是运用FISH技术还是IHC技术进行检测均具有较高的检出率，而其中导致两种检测技术结果出现偏差的因素在于17号染色体多体的发生率，临床中对于乳腺癌HER-2基因扩增检测可应用FISH技术进行操作，值得推广应用。

    【关键词】FISH技术；乳腺癌HER-2基因；IHC技术；17号染色体

    Analysis of the application value of FISH technology in breast cancer HER-2 gene detection

    FEI Bingyou

    Huai an Maternal and Child Health Care Hospital， Huaian， Jiangsu 223002， China

    【Abstract】Objective：To explore the application value of FISH technology in HER-2 gene detection in breast cancer.Methods：42 breast cancer patients from August 2018 to August 2021were selected for retrospective analysis. All samples collected from the 42 patients were tested for their HER-2 gene by FISH and IHC and compared the positive compliance rate of different testing techniques to observe the detection of chromosome 17 by FISH.Results：Theconsistencyof different detection techniques was relatively high (Kappa=0.544 ......