【摘要】目的:建立一种可测定小儿肺热咳喘口服液中黄芩苷的RP-HPLC方法。方法:采用Shim-pack VP-ODS(250mm×4.6mm5μm)为色谱柱;流动相为甲醇-0.3%磷酸溶液(42:58);检测波长为277nm;流速为1.0mL/min;柱温为40℃;峰面积外标法定量;结果:本法黄芩苷在1.044×10-2μg～2.61μg范围内呈良好的线性关系，(r=0.999995)，平均回收率为99.0%，RSD0.99%;结论:本方法简便、快速、准确可靠，可作为该制剂的质控方法。

    【关键词】小儿肺热咳喘口服液;黄芩苷;高效液相色谱法;含量测定

    【中图分类号】R917【文献标识码】A【文章编号】1007-8517(2010)12-107-2

    Determination of baicalin in Xiao’er feire kequan Oral Liquid by RP-HPLC

    ZHANGzhi-geng1SONGJian-feng2LIN na3

    Quzhou Institute for Drug Control，Quzhou，China 324002

    ABSTRACT:AIM:To establish a RP-HPLC method for determining the content of baicalin in Xiao’er feire kequan Oral Liquid.METHODS:A Shim-pack C_(18)column was used.The mobile phase wasmethanol-0.3%Phosphoric acid solution(42:58).The detection wavelength was 277nm. RESULTS:The linear range of baicalin was1.044×10-2μg～2.61μg(r=0.999995).The average recovery(n=9) was 99.0%.RSD was 0.99%. CONCLUSION:The method is simple ......