【摘要】目的：为了研究乳腺癌发生的分子遗传学机理，分离克隆模型鼠乳腺癌组织中特异表达的基因。方法：利用抑制消减杂交技术，结合反向cDNA斑点杂交试验，小鼠乳腺癌组织中分离出与正常乳腺组织差异表达的EST，应用生物信息学方法鉴定所得EST的小鼠同源基因，并分析其与乳腺癌发生的相关性。结果：获得20个在小鼠乳腺癌组织中高表达的，与已知的小鼠基因片段高度同源的基因，及2个与数据库中提交的假想蛋白基因高度同源的基因。结论：利用抑制消减杂交技术，可以高效高通量分析在乳腺癌中特异表达的基因，探讨乳腺癌发生的分子机制。

    【关键词】抑制消减杂交技术；乳腺癌；基因

    【中图分类号】R737.9【文献标识码】A【文章编号】1007-8517(2009)10-0021-02

    Screening Specific Expressed Genes of Breast Cancer by SSH

    WANG YanZGHONGShijunSHAO MinZHANG Qingfeng

    (ZUNYi Medical College School of Zhu Hai ,GUANG Dong,Zhu Hai ,519041)

    【Abstract】 Objective In order to study the molecular mechanism of breast cancer, to screen the genes differentially expressed in breast cancer. Methods Suppression subtractive hybridization (SSH)combine reverse dot blotting was performed to isolate the differentially expressed genes between breast cancer and normal mammarytissues. A subtractive cDNA library of highly expressed genes in breast tumor tissue was established. Result About 22 differentiallyexpressed genes were obtained. By sequencing and BLAST analysiswe found 2 genes are new genes. Conclusion SSHis an effective method with high specificity to detect specific expressed genes in breastcancer genesisanddevelopment. ......