安徽省蚌埠市蚌埠医学院第一附属医院，安徽 蚌埠 233000

    不同长春花中3种生物碱的含量测定

    詹小宁

    安徽省蚌埠市蚌埠医学院第一附属医院，安徽 蚌埠 233000

    目的建立快速准确的长春花内生物碱含量测定方法，为寻找高含量品种提供方法和依据。方法采用反相高效液相色谱法(RP-HPLC)，对收集到的10个长春花品种中的文多灵、长春质碱、长春碱含量进行了测定，色谱柱：Dikma Diamonsil-C18( 4.6mm ×250mm, 5μm)；流动相为A∶0.7%二乙胺(磷酸调整pH至7.2)；B：乙腈：甲醇=25：40。A∶B=35∶65；流速1ml/min。柱温35℃。检测波长：280nm。结果文多灵：Y=2.00×106X+5.46×104，r=0.9999，线性范围：0.2～4μg；长春质碱：Y=2.28×106X+8.31×104，r=0.9999，线性范围：0.2～4μg；长春碱：Y=1.76×106X-7.17×104，r=0.9999，线性范围：0.2～4μg。文多灵含量最高的是1号品种，长春质碱含量最高的是3号品种，长春碱含量最高的是2号品种，三种生物碱总和最高的是3号品种。结论该方法简便、快速，分析准确，适合对长春花内主要生物碱做高通量的筛选。综合考虑总碱的含量和最重要的长春碱的含量，1号品种适合作为代谢调控的本体。

    长春花；长春碱；长春质碱；文多灵；RP-HPLC

    Abstract:ObjectiveTo establish a fast and accurate method of determination of alkaloids inCatharanthusroseusfor finding high-content varieties.MethodTo determinate the contents of Vinblastine, Catharanthine and Vindoline in the 10 varieties ofCatharanthusroseusby RP-HPLC,HPLC method is performed on Dikma Diamonsil - C18(4.6mm×250mm, 5μm)column, mobile phase:A:0.7% diethylamine(pH 7.2 adjusted by phosphoric acid );B:acetonitrile∶methanol =25∶40.A∶B = 35∶65; flow rate is 1ml/min.Column temperature is 35 ℃. Detected at 280nm.ResultsVindoline:Y=2.00×106X+5.46×104,r=0.9999,linear range:0.2 ～ 4μg;Catharanthine:Y=2.28×106X+8.31×104,r=0.9999,linear:0.2 ～ 4μg;Vinblastine:Y=1.76×106X-7.17×104,r=0.9999,linear range:0.2～ 4μg.The species of the highest content of vindoline is the 1st, Catharanthine is the 3rd, vinblastine is the 2nd species, the highest sum of three alkaloids is on the 3rd varieties.ConclusionThis method is simple, reliable, repeatable, and is suitable for High-throughput screening of the Alkaloids content inCatharanthusroseus. The 1st varieties is suitable as the body of metabolic regulation. ......