【摘要】目的：建立同时测定壮阳类中成药和保健品中可能添加的5种5型磷酸二酯酶(PDE5)型抑制剂UPLC-MS/MS分析方法，方法：采用液质联用法 ，应用C18柱，流动相为含0.1%甲酸水溶液和乙腈梯度洗脱，质谱采用正离子模式，多反应监测扫描。 结果：5种PDE5 型抑制剂线性关系、精密度均符合方法学测定要求，加标回收率为80.8%～101.9%。结论：该法操作简便、快捷、高灵敏度、高准确性等特点，可同时对中成药和保健食品中非法添加多种PDE5 型抑制剂进行定性定量分离检测。

    【关键词】UPLC-MS/MS；壮阳类中成药；保健食品；PDE5 型抑制剂

    【中图分类号】R284.1 【文献标志码】A 【文章编号】1007-8517(2016)07-0021-02

    Abstract：Objective To establish a UPLC-MS/MS analytical method for the simultaneous analysis of five PDE5 inhibitors in Chinese trandition medicines and Health Food.Methods The separation was performed on C18 column using a gradient elution(0.1% formic acid in water and acetonitrile). A triple quadrupole mass spectrometer operating in positive electrospray ionization mode with multiple reaction monitoring was used.Results five PDE5 inhibitors have good linear relationship and precision according to the requirements of the methodology determination. The recoveries were 80.8%～101.9%.Conclusion the method not only isconvenient ......