【摘 要】目的：建立高效液相色谱法测定藏药石榴莲花散中鞣花酸和胡椒碱含量的方法。方法：采用HPLC法对制剂中石榴子和诃子的鞣花酸进行含量测定;采用HPLC法对制剂中荜茇的胡椒碱进行含量测定。结果：鞣花酸在5.065～25.325 μg呈良好线性关系(r=0.9999)，平均回收率为98.2%，RSD=2.8%(n=6);胡椒碱在10.414～52.071 μg呈良好线性关系(r=1.0000)，平均回收率为99.4%，RSD=1.1%(n=6)。结论：该方法简便、准确、重复性好，专属性高，结果可靠，可有效控制石榴莲花散中鞣花酸和胡椒碱成分的质量。

    【关键词】石榴莲花散;鞣花酸;胡椒碱;含量测定;高效液相色谱法

    【中图分类号】R284.1 【文献标志码】A 【文章编号】1007-8517(2019)19-0037-05

    Abstract： Objective To establish a HPLC method for the determination of ellagic acid and piperine in Tibetan medicine garneta powder. Methods The content of ellagic acid in pomegranate and terminalia fruit was determined by HPLC. HPLC method for preparation of piper Ba piperine content determination. Results Ellagic acid showed a good linear relationship at 5.065 ～ 25.325 μg (r=0.9999) ......