蛋白激酶A在TGF-β1刺激增生性瘢痕成纤维细胞胶原合成中的

蛋白激酶A在TGF-β1刺激增生性瘢痕成纤维细胞胶原合成中的作用

http://www.100md.com 2001年2月1日《中国美容医学》 2001年第1期

     张选奋男，1965年生。兰州医学院副教授、副主任医师。现在第四军医大学西京医院整形外科中心从师李荟元教授和鲁开化教授攻读博士学位。已发表论文16篇。

    [摘要]目的：探讨蛋白激酶A在TGF-β₁刺激增生性瘢痕和正常人皮肤成纤维细胞(HS-FB和NS-FB)合成胶原中的信号转导作用。方法：利用³²P掺入底物法测定TGF-β₁刺激的HS-FB和NS-FB的PKA活性，³ H-脯氨酸掺入法和放射免疫法测定胶原合成能力。结果：NS-FB被TGF-β₁刺激后PKA的活性短暂升高后很快恢复，HS-FB则在30～60min降低(P<0.05)。TGF-β₁对两种细胞有加速合成胶原的作用(30min后P<0.05)，HS-FB的合成能力比NS-FB强(刺激60min后P<0.05)。cAMP有抑制作用(60min后P<0.05)，H7则有拟TGF-β₁作用(与对照比较时30min后P<0.05)，而H7可增强TGF-β₁刺激的作用(30～60min时P<0.05)。结论：TGF-β₁刺激两种细胞后PKA的活性变化提示cAMP/PKA通道参与介导TGF-β₁信号：TGF-β₁对HS-FB的短期刺激作用与PKA活性降低有关，但长期刺激作用与PKA通道活性变化无关；cAMP/PKA活化可以抑制FB合成胶原。[关键词]信号转导蛋白激酶A转化生长因子β₁成纤维细胞胶原合成

    [中图分类号]R619+.6[文献标识码]A[文章编号]1008-6455(2001)01-0009-04

    THE EFFECTS OF ACTIVITY OF PROTEIN KINASE A IN TGF-β₁ STIMULATING

    COLLAGEN SYNTHESIS OF NORMAL SKIN AND HYPERTROPHIC SCAR FIBROBLASTS

    ZHANG Xuan-fenLU Kai-huaLI Hui-yuan et al

    Center of Plastic Surgery,Xijing Hospital,Fourth Military Medical University.(Xian,Shanxi 710032)

    [Abstract]Objective：To investigate the effects of protein kinase A(PKA)in TGF-β₁ stimulating collagen synthesis of normal skin and hypertrophic scar fibroblast(NS-FB and HS-FB).Methods：The activity of PKA of HS-FB and NS-FB was detected by 32P incorporation assay.Total collagen synthesis was measured by ³H-proline incorporation assay and radioimmunoassay.Results：The activity of PKA of NS-FB rose temporarily insigmificantly and recovered soon while the ones of HS-FB decreased and recovered at lh the stimulation of TGF-β₁(P<0.05).TGF-β₁ could enhance collagen synthesis of FB(P<0.05 at stimulating 30 minutes later) but the effects were stronger in HS-FB(〖WTBX〗P<0.05 at stimulating 60 minutes later).cAMP could inhibit the changes (P<0.05 at stimulation 60 minutes) while H7 could improve roles of TGF-β₁(P<0.05 at 30～60minutes of stimulation).Conclusion：The changes of the PKA activity of the two types of cell after the stimulation of TGF-β₁ might implicate that there was difference in the PKA signal pathway.The effects of TGF-β₁ stimulating collagen synthesis might have partly relation to cAMP/PKA pathway but activating cAMP/PKA signal pathway could abrogate FB to synthesize collagen. ......

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