CyclinD1和P21在肥大乳房腺体组织中的表达及意义(1)
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[摘要]目的：探讨肥大乳房中CyclinD1和P21的表达级意义。方法：采用免疫组化SP法，分别测定了34例腺性肥大乳房和30例正常体积乳房腺体中CyclinD1和P21的表达。结果：CyclinD1在34例肥大乳房中，阳性18例，阳性率52.94%；30例正常对照组中，全部表达阴性，阳性率0，两组间比较具有统计学意义(P<0.05)。P21在34例肥大乳房中，3例阳性，阳性率8.82%；正常对照组30例，阳性9例，阳性率30.00%。两组间比较具有统计学意义(P<0.05)。结论：乳房肥大的形成可能与CyclinD1增多和P21减少有关。
[关键词]CyclinD1;P21;乳房肥大
[中图分类号]R730.45[文献标识码]A[文章编号]1008-6455(2011)10-1556-02
Expression of CyclinD1 and P21 in the mammary gland of the hypertrophic breast and their significance
JIN Jun-hua,ZHANG Rui-rui,WANG Qi,XIE Qun,LIU Dan-dan,HUI Zu-yan,HU Qiang
(Department of Plastic Surgery,the Central Hospital of Xiangyang,Xiangyang 441021,Hubei,China)
AbstractObjectiveTo detect the expression of CyclinD1 and P21 in the mammary gland of the hypertrophic breast and the normal voluminal breast,so that their function and significance in the pathogenesis of breast hypertrophy can be investigated.MethodsThe breast tissue samples were obtained, including hypertrophic breast tissue(34 cases) and normal voluminal breast tissue(30 cases).They were detected by the SP method immunohistochemistry for the expression of CyclinD1 and P21.ResultsThe positive expression rate of CyclinD1 in the hypertrophic breast was 52.94%(18 of 34),while 0 in the normal voluminal breast(0 of 30),showing a significant difference between the two groups(P<0.05). The positive expression rates of P21 in the hypertrophic breast and normal voluminal breast were 8.82%(3 of 34) and 30.00%(9 of 30), showing a significant difference between the two groups(P<0.05). ConclusionThe development of hypertrophic breast may be related to the excessive expression of CyclinD1and decreased expression of P21 in the breast tissue.
Key words: CyclinD1;P21; hypertrophic breast
乳房肥大是一种常见的良性增生性病变，其主要表现为乳房体积过度增大，常给患者带来身心困扰。其确切的发病机制目前尚不明确，本课题通过免疫组化的方法，检测了34例腺性肥大乳房和30例正常体积乳房腺体中CyclinD1和P21的表达，并对结果进行统计学分析，旨在探讨CyclinD1和P21在乳房肥大症发病机制中的作用。
1材料和方法
1.1材料
1.1.1标本：实验组选自2007年7月～2011年6月就诊于我科的肥大乳房患者34例，同期收治的因单纯乳房下垂行乳房悬吊固定术的患者30例作为对照组。其中肥大乳房患者单侧乳房体积均在600g以上，对照组乳房体积在250～350g。实验组年龄18～45岁，平均年龄28.33岁；对照组年龄26～46岁，平均年龄31.21岁。两组间年龄经统计学分析无统计学意义(采用两样本t检验，P>0.05)。
1.1.2试剂：一抗为鼠抗人P21, CyclinD1单克隆抗体，购自美国Santa Cruz公司；SABC免疫组化试剂盒，购自美国New Mark公司。
1.2方法
1.2.1 实验方法：标本经4%的福尔马林固定，石蜡包埋 ......
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