阿司匹林对正常人表皮黑素细胞系PIG1细胞黑素生成的影响(1)
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[摘要]目的：观察阿司匹林对体外培养的正常人表皮黑素细胞系PIG1细胞的活力、黑素生成及酪氨酸酶活性的影响。方法：用不同浓度的阿司匹林处理PIG1细胞72h，CCK-8比色法测定阿司匹林对PIG1细胞活力的影响；光学显微镜观察细胞形态学变化；氢氧化钠裂解法测定黑素的含量；体外多巴氧化反应法测定酪氨酸酶活性的变化。结果：阿司匹林浓度小于或者等于500μmol/L时无明显细胞毒性；浓度大于或者等于125μmol/L时以剂量依赖方式抑制黑素生成，差异有统计学意义(P<0.05)；黑素细胞酪氨酸酶活性无显著性变化(P>0.05)。结论：阿司匹林抑制PIG1细胞的黑素生成，可能应用于色素增多性皮肤病。
[关键词]阿司匹林；黑素细胞；黑素生成；酪氨酸酶
[中图分类号]Q813.1 [文献标识码]A [文章编号]1008-6455(2012)04-0593-03
Effects of aspirin on melanogenesis of human epidermal melanocyte cell line PIG1 cells in vitro
TANG Ling-zhen,JIAN Zhe,LIU Bang-min,LI Kai,LI Qiang,LI Chun-ying,GAO Tian-wen
(Institute of Dermatology,Xijing Hospital,Fourth Military Medical University,Xi'an 710032, Shaanxi,China)
Abstrat: Objective To investigate the effects of aspirin on cell viability, tyrosinase activity and melanogenesis of human epidermal melanocyte cell line PIG1 cells in vitro. Methods After treating PIG1 cell with aspirin in different concentrations for 72h, we employed CCK-8 assay, oxidative DOPA reaction and NaOH method to measure the cell viability, tyrosinase activity and melanogenesis of PIG1 cells respectively. The morphologic changes of PIG1 cells were observed via light microscope. Results Aspirin had no significant effect on the cell viability of PIG1 cells when the concentrations were lower than 500μmol/L. The melanogenesis of PIG1 cells was remarkably decreased in a concentration-dependent manner by aspirin at the concentrations higher than 125μmol/L (P<0.05). However the tyrosinase activity had no significant change after aspirin treatment (P>0.05). Conclusion Aspirin can inhibit the melanogenesis of PIG1 cells, thus it may be developed as a clinical treatment of hyperpigmented skin diseases.
Key words: aspirin; melanocyte; melanogenesis; tyrosinase
人皮肤颜色的深浅主要由表皮中黑素细胞生成的黑素量决定，抑制黑素生成一直是皮肤美白及治疗色素增多性疾病的主要策略。酪氨酸经过一系列氧化反应生成黑素的过程中，需要活性氧的参与[1]，因此，抗氧化剂通常能减少活性氧来抑制黑素的生成[2]。阿司匹林是临床常用的非甾体类抗炎药(NSAIDs)，具有解热、镇痛、抗炎等作用。近年来有研究证实阿司匹林具有清除自由基及抗氧化作用[3-4]。有研究发现阿司匹林能降低小鼠B16黑素瘤细胞的酪氨酸酶表达[5]，但阿司匹林对正常人黑素细胞黑素生成及酪氨酸酶活性的影响还未有研究报道。本研究探讨了阿司匹林对体外培养的正常人表皮黑素细胞系PIG1细胞的黑素生成及酪氨酸酶活性的影响，为其应用于色素增多性皮肤病的预防和治疗提供实验依据。
1 材料和方法
1.1 材料 ......
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