胰岛素样生长因子—1对猪关节软骨细胞糖胺多糖的作用(1)
[提要]目的：探讨胰岛素样生长因子-1(IGF-1)对软骨细胞分泌糖胺多糖的作用。方法：通过Ⅱ型胶原酶消化法获得高活性的软骨细胞，定量接种5×106个细胞于培养皿，在传代细胞培养皿中加入100 μg/L 的IGF-1，每周传代1次，连续4周，留取培养液，用阿利新蓝法(Alcian Blue)测定软骨细胞糖胺多糖(GAG)的变化；利用光镜观察原代及传代软骨细胞的生长情况。结果：软骨细胞从传代后开始合成糖胺多糖，第二代传代细胞分泌GAG的能力最强； 浓度为100μg/ml的IGF-1能明显促进传代软骨细胞合成糖胺多糖。结论：IGF-1能明显促进体外培养的传代软骨细胞合成大量的糖胺多糖。
[关键词]软骨细胞；细胞培养；胰岛素样生长因子；糖胺多糖
[中图分类号]Q813.1 [文献标志码]A [文章编号]1008-6455(2015)09-0034-03
Abstract： Objective To explore the effect of Insulin-like growth factor 1(IGF-1) on the synthesis of glycosaminoglycan(GAG) in pig articular chondrocytes. Methods The articular cartilage of pig were digested by collagenase II and the chondrocytes were collected ......
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[关键词]软骨细胞；细胞培养；胰岛素样生长因子；糖胺多糖
[中图分类号]Q813.1 [文献标志码]A [文章编号]1008-6455(2015)09-0034-03
Abstract： Objective To explore the effect of Insulin-like growth factor 1(IGF-1) on the synthesis of glycosaminoglycan(GAG) in pig articular chondrocytes. Methods The articular cartilage of pig were digested by collagenase II and the chondrocytes were collected ......
您现在查看是摘要页，全文长 3322 字符。