詹明峰 张文娟 孙士芳 尚佩生 沈晓峰

    [摘要]目的：探討长链非编码RNA 8975-1(Long non-coding RNA 8975-1，LNCRNA8975-1)通过微小RNA-203(microRNA-203，miR-203)/血管内皮生长因子-A(Vascular endothelial growth factor-A， VEGF-A)信号轴对人瘢痕疙瘩成纤维细胞(Human keloid fibroblast， HKF)的细胞增殖周期和迁移的影响及其机制。方法：培养人皮肤成纤维细胞(Human skin fibroblasts，HSF)和HKF，采用定量PCR(quantitative polymerase chain reaction， qPCR)及western blot法检测LNCRNA8975-1、miR-203和I型胶原蛋白(Type I collagen， Col-I)的表达量。将HKF细胞分为Control组、小干扰RNA载体的阴性对照(the negative control for small interfering RNA vector， siNC)组、沉默LNCRNA8975-1的小干扰RNA载体(the small interfering RNA vector for silence of LNCRNA8975-1， siLNCRNA8975-1)组、pcDNA3.1空载体(the empty pcDNA3.1 vector， pcDNA)组、过表达LNCRNA8975-1的pcDNA3.1载体(overexpression of LNCRNA8975-1 in the pcDNA3.1 vector， pcDNA-LNCRNA8975-1)组、miR-203拟似物的阴性对照(the negative control of miR-203 mimic ......