慢性束缚应激对大鼠血清皮质酮和前额叶皮质p-CREB表达的影响(1)

http://www.100md.com 2011年4月1日 孟珊珊 刘洪文 贾非

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     【摘要】目的：探讨慢性束缚应激对大鼠血清皮质酮水平和前额叶皮质p-CREB表达的影响。方法：将30只雄性SD大鼠随机分为对照组，慢性束缚应激1周组和2周组。比较应激前后大鼠血清皮质酮水平和前额叶皮质p-CREB表达的变化。结果：束缚应激1周组和2周组大鼠血清皮质醇浓度分别为(356.78±112.21) ng/ml、(558.49±98.32) ng/ml，均高于对照组(198.21±101.35)ng/ml)，有统计学差异。束缚应激1周组(3.78±0.12)和2周组(2.39±0.08)大鼠前额叶皮质的p-CREB水平低于对照组(6.08±0.19)，p<0.01。结论：慢性束缚应激引起大鼠血清皮质酮水平增高和前额叶皮质p-CREB水平降低。

    【关键词】束缚应激；皮质酮；p-CREB

    【中图分类号】R285.5【文献标识码】A【文章编号】1008-6455(2011)08-0020-02

    Effect of chronic restraint stress on serum corticosterone concentration and expression of prefrontal cortex p-CREB in the rat

    Meng Shanshan₁ Liu Hongwen₁ Jia Fei₂

    【Abstract】Objective：To observe the change of serum corticosterone concentration and prefrontal cortex p-CREB expression in the rat induced by chronic restraint stress. Method Thirty male SD rats were randomly divided into control group, 1 week and 2 weeks restraint stress groups. Before and after restraint stress, the changes of serum corticosterone concentration were examined, and the level of p-CREB in the prefrontal cortex was analyzed by Western blotting. Results The serum corticosterone level in 1 week [(356.78±112.21) ng/ml] and 2 weeks [(558.49±98.32) ng/ml] restraint stress groups were markedly higher than that in control group[(198.21±101.35) ng/ml, P<0.05]. The p-CREB level in prefrontal cortex in 1 week (3.78±0.12) and 2 weeks (2.39±0.08) restraint stress groups were markedly lower than that in control group(6.08±0.19, P<0.01). Conclusion Chronic restraint stress significantlyincreases serum corticosterone level and reduces p-CREB level in prefrontal cortex of rat.

    【Key words】Restraint stress; Corticosterone; p-CREB

    机体长时间处于应激状态下会导致抑郁症的发生^[1]。慢性束缚应激作为一种慢性应激模型，已被广泛的应用到应激相关的精神疾病基础研究中^[2]。我们采用慢性束缚应激模型，观察应激后大鼠的血清皮质酮和前额叶皮质环磷酸腺苷反应元件结合蛋白(p-CREB)磷酸化水平的变化，为探索应激发生的分子病理机制提供线索。

    1 材料与方法

    1.1 材料：雄性SD大鼠30只，体重180-200g，购自哈尔滨医科大学动物中心。动物置于自然昼夜节律光照条件下，自由进食水，每笼2-3只，控制室温(22±2)℃。动物随机分为对照组，慢性束缚应激1周组和慢性束缚应激2周组，每组10只。所用试剂均购于Sigma公司。

    1.2 方法

    1.2.1 建立模型：对照组不做任何应激处理,仅在应激组应激阶段禁食水；慢性束缚应激组将大鼠置于束缚管中，确保通风良好，大鼠在束缚管中有限的空间活动，但不会造成肉眼可见的躯体上的伤害。动物每日应激6h，连续进行1周和2周，应激阶段禁食水 ......

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