论文下载：蜕皮甾酮对氧化损伤的人晶状体上皮细胞凋亡的防护作用

蜕皮甾酮对氧化损伤的人晶状体上皮细胞凋亡的防护作用(1)

http://www.100md.com 2010年4月1日黄秀榕1,祁明信2,郭娜1,张可丽1

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    参见附件。

     (1.福建中医学院病理生理研究中心,福州福建 350003;

    2.福建中医学院附属第二人民医院眼科,福州福建 350003)

    收稿日期:2009-11-07

    作者简介:黄秀榕(1948-),女,福建福州人,教授,博士研究生导师,主要从事白内障的基础与临床研究。

    摘要:目的:探讨蜕皮甾酮(ECR)对氧化损伤的人晶状体上皮细胞(HLEC-B₃)凋亡有无抑制作用。方法:本研究在终浓度300μmol/L H₂O₂培养液中复制HLEC凋亡模型,并以雌二醇(E₂)作为阳性对照,在透射电子显微镜下观察HLEC-B₃超微结构的改变,采用FCM法检测HLEC-B₃凋亡率及线粒体膜电位的变化。结果:超微结构研究显示,H₂O₂组绝大多数HLEC-B₃发生凋亡,并呈凋亡各期改变的全过程;E₂、ECR组仅少数HLEC-B₃发生凋亡,并多为早期或中期的轻微改变。FCM结果显示:H₂O₂组HLEC凋亡率显著高于空白对照组;E₂和ECR组凋亡率均低于H₂O₂组(P<0.01)。H₂O₂组线粒体跨膜电位比对照组显著下降;E₂组和ECR组的线粒体跨膜电位均比H₂O₂组升高(P<0.01)。结论:ECR可减轻实验性氧化损伤的HLEC凋亡程度,为寻求防治老年性白内障的药物提供实验依据。

    关键词:抗氧化药;蜕皮甾酮;白内障;凋亡

    中图分类号:R285.5 文献标识码:A 文章编号:1673-7717(2010)04-0692-04

    Ecdysterone Protecting Against Apoptosis of Human

    Lens Epithelial Cell Injured By Oxidation

    HUANG XiuRong1,QI MingXin2,GUO Na1,ZHANG Keli1

    (1.The Research Center of Pathophysiology,Fujian College of Traditional Chinese Medicine,Fujian 350003,Fuzhou,China

    2.The Second Affiliated People’s Hospital,Fujian 350003,Fuzhou,China)

    Abstract:Objective:To investigate if Ecdysterone inhibits the apoptosis of human lens epithelial cell resulted from the experimental oxidative injury.Methods:Lens were incubated with 300μmol/LH₂0₂.And make estradiol (E₂) as a positive control,HLEC-B₃ apoptosis and apoptosis rate were measured by FCM, Ultrastructure changes and apoptosis bodies of HLEC-B₃ were observed under transmission electron microscope.Results:The results showed:①Ultrastructure observation indicated that apoptosis cells occurred in most HLEC-B₃ of H₂0₂ group and the changes were severe and presenting different stages ......

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