电针对局灶性脑缺血模型大鼠皮层BDNF、TrkB的影响(1)
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摘要：目的：观察电针对不同时间点局灶性脑缺血模型大鼠缺血区皮层BDNF、TrkB的影响。方法：采用线栓改良法复制大脑中动脉缺血模型(MCAO)，采用免疫组化SABC法观察缺血皮层BDNF、TrkB表达情况。结果：BDNF、TrkB阳性神经元主要分布在梗死灶周围皮质区。假手术组大鼠相应区域可见少量阳性神经元表达，且强度较弱；模型组各时间点梗死周围皮质BDNF、TrkB阳性神经元比假手术组明显增多，差异有统计学意义(P<005)；电针组各时间点梗死周围皮质BDNF、TrkB比相同时间点模型组显著增多，差异有统计学意义(P<005)。结论：电针风府穴可以增加急性脑缺血后缺血周围皮质BDNF、TrkB阳性神经元，对缺血性脑损伤起修复和保护作用。
关键词：脑缺血；电针；风府穴；脑源性神经营养因子(BDNF)；酪氨酸激酶B(TrkB)
中图分类号：R285.5；R245.97文献标识码：A文章编号：1673-7717(2012)03-0469-03
Influence of Electroacupuncture on the Expressions of BDNF and
TrkB in Ischemic Cortex of Rats With Local Cerebral Ischemia
WANG Yanchun1,LIANG Shaorong1，MA Jun1,GNA Shuiyong1,WANG Shuju1,JIANG Chengkun1,LEI jun1,WANG Peijun1,XU Yonghai1,CHEN Jianming2
(1.Hubei University of Traditional Chinese Medicine,Wuhan 430061,China;
2.Beijing University of TCM,Beijing 100029,China)
Abstract:Objective:To observe the influence of electroacupuncture(EA)on the expressions of BDNF and its receptor TrkB in ischemic cortex of rats with local cerebral ischemia. Methods:The model of focal cerebral ischemia was set up by blocking up the middle cerebral artery(MCA) with filament in rats.The expressions of BDNF and TrkB positive neurons in ischemic cortex was observed with the method of immunocytochemistry SABC. Results:BDNF and TrkB positive neurons were mainly distributed in cortex around the ischemic focus. In pseudo-operation groups ,BDNF and TrkB positive neurons were few and low-expression in identical area.The expressions of BDNF and TrkB around ischemic cortex of model groups were significantly higher than that of pseudo-operation group(P<0.05); BDNF and TrkB positive neurons in EA group were more than that of model group(P<0.05).Conclusion:EA at Fengfu has a protective effect on ischemic brain injury by increasing the BDNF and its receptor TrkB expressions in cortex around the ischemic focus.
Key words:cerebral ischemia;electroacupuncture;fengfu point;BDNF;TrkB
收稿日期：2011-11-27
基金项目：国家自然科学基金资助项目(30300461,30973787,30973809)；湖北省教育厅科学技术研究资助项目(2002B00004，D200726001，Z20091601)；湖北省科技厅科学技术资助项目(2009CDA068，2010CDA101)；武汉市科学技术局科学技术研究资助项目(201060623269) ......
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