庞琼，胡志立(.郴州市疾病预防控制中心体检科，湖南423000；2.湘南学院胃肠外科，湖南郴州423000)

    ·论著·

    溪黄草抗乙型肝炎病毒体外抑制作用研究

    庞琼1，胡志立2△

    (1.郴州市疾病预防控制中心体检科，湖南423000；2.湘南学院胃肠外科，湖南郴州423000)

    目的为证实溪黄草单味药具有体外抗乙型肝炎病毒活性提供重要的科学依据。方法将溪黄草50%乙醇提取物配置成一定药物浓度，拟采用HepG2.2.15细胞模型进行细胞毒试验和乙型肝炎表面抗原(HBsAg)、乙型肝炎e抗原(HBeAg)酶联免疫检测。结果半数细胞抑制时其浓度为4.275mg/mL，溪黄草粗提物对HBsAg的半抑制浓度(IC50)为2.250mg/mL，对HBeAg的IC50为2.150mg/mL，治疗指数小于2。结论溪黄草粗提物具有抑制体外乙型肝炎病毒的作用。

    溪黄草；肝炎病毒，乙型；细胞培养技术；植物，药用；细胞毒性试验，免疫

    【Abstract】Objective To provide an important scientific basis for verifying the in vitro anti-HBV activity of single herb Rabdosia Serra.M ethods 50% ethanolextract of Rabdosia Serra was dispensed ascertain concentrations.The HepG 2.2.15 cellularmodel was adopted to conduct the cellular toxicity testand HBs Ag and HBe Ag enzyme-linked immunosorbentssay(ELISA). Results IC50was 4.275mg/mL，IC50of crude extract of Rabdosia Serra on HBs Ag was 2.250mg/mL，which on HBe Ag was 2.150 mg/mL，the therapeutic index(TI)<2.Conclusion The crude extract of Rabdosia Serra has the effect for in vitro inhibiting HBV.

    【Key words】Isodon striatus；Hepatitis B virus；Cell culture techniques；Plants ......