乳腺癌中NDRG-1基因甲基化状态研究(3)
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对于NDRG-1基因的启动子区甲基化状态研究，国内外尚无研究报道。本实验利用双色荧光杂交微阵列基因芯片技术对NDRG-1基因启动子区甲基化水平进行定量检测，发现CpG位点肿瘤组织和对应癌旁组织的甲基化率分别为40.76%和22.41%，差异有统计学意义。因此可以推测甲基化调控机制可能是NDRG-1基因的重要调控途径之一，从而与乳腺癌的发生相关。
[参考文献]
[1]van Belzen N,Dinjens W N,Diesveld M P,et al.A novel gene which is up-regulated during colon epithelial cell differentiation and down-regulated in colorectal neoplasms [J].Lab Invest,1997，77(1):85-92.
[2]Kurdistani S K,Arizti P,Reimer C L,et al.Inhibition of tumor cell growth by RTP/ rit42 and its responsiveness to p53 and DNA damage [J].Cancer Res,1998，58(19):4439-4444.
[3]Zhou D,Salnikow K,Costa M.Cap43,a novel gene specifically induced by Ni2+ compounds [J].Cancer Res,1998，58(10):2182-2189.
[4]Park H,Adams M A,Lachat P,et al.Hypoxia induces the expression of a 43-kDa protein(PROXY-1)in normal and m alignant cells[J].Biochem Biophys Res Commun,2000，276(1)：321-328.
[5]Shimono A,Okuda T,Kondoh H.N-myc-dependent repression of ndr1,a gene identified by direct subtraction of whole mouse embryo cDNAs between wild type and N-myc mutant [J].Mech Dev,1999，83(1-2):39-52.
[6]Wang Z,Liu Q,Chen Q,et al.Overexpression of NDRG1:relationship with proliferative activity and invasiveness of breast cancer cell line and breast cancer meta stasis [J].Zhonghua Bing Li Xue Za Zhi,2006，35(6):333-338.
[7]Zhou D,Qiao W,Wan Y,et al.Microarray-based methylation analysis using dual-color fluorescence hybridization[J].J Biochem Biophys Methods,2006,66(1-3):33-43.
[8]Frommer M,McDonald L E,Millar D S,et al ......
对于NDRG-1基因的启动子区甲基化状态研究，国内外尚无研究报道。本实验利用双色荧光杂交微阵列基因芯片技术对NDRG-1基因启动子区甲基化水平进行定量检测，发现CpG位点肿瘤组织和对应癌旁组织的甲基化率分别为40.76%和22.41%，差异有统计学意义。因此可以推测甲基化调控机制可能是NDRG-1基因的重要调控途径之一，从而与乳腺癌的发生相关。
[参考文献]
[1]van Belzen N,Dinjens W N,Diesveld M P,et al.A novel gene which is up-regulated during colon epithelial cell differentiation and down-regulated in colorectal neoplasms [J].Lab Invest,1997，77(1):85-92.
[2]Kurdistani S K,Arizti P,Reimer C L,et al.Inhibition of tumor cell growth by RTP/ rit42 and its responsiveness to p53 and DNA damage [J].Cancer Res,1998，58(19):4439-4444.
[3]Zhou D,Salnikow K,Costa M.Cap43,a novel gene specifically induced by Ni2+ compounds [J].Cancer Res,1998，58(10):2182-2189.
[4]Park H,Adams M A,Lachat P,et al.Hypoxia induces the expression of a 43-kDa protein(PROXY-1)in normal and m alignant cells[J].Biochem Biophys Res Commun,2000，276(1)：321-328.
[5]Shimono A,Okuda T,Kondoh H.N-myc-dependent repression of ndr1,a gene identified by direct subtraction of whole mouse embryo cDNAs between wild type and N-myc mutant [J].Mech Dev,1999，83(1-2):39-52.
[6]Wang Z,Liu Q,Chen Q,et al.Overexpression of NDRG1:relationship with proliferative activity and invasiveness of breast cancer cell line and breast cancer meta stasis [J].Zhonghua Bing Li Xue Za Zhi,2006，35(6):333-338.
[7]Zhou D,Qiao W,Wan Y,et al.Microarray-based methylation analysis using dual-color fluorescence hybridization[J].J Biochem Biophys Methods,2006,66(1-3):33-43.
[8]Frommer M,McDonald L E,Millar D S,et al ......
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