全蝎纯化液对实验性动脉血栓形成t-PA、PAI-1的影响(1)
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摘要：目的 观察全蝎纯化液对新西兰白兔颈总动脉血栓形成组织型纤溶酶原激活物(tPA)、纤溶酶原活化物抑制剂(PAI1)、血小板计数(Pt)的变化。方法 将50只健康新西兰白兔随机分成5组：空白组、模型组、全蝎纯化液大剂量组(20 mg/kg)、全蝎纯化液中剂量组(10 mg/kg)和全蝎纯化液小剂量组(5 mg/kg)。耳缘静脉注射药物(空白组、模型组注射同等体积的生理盐水)后，采用H2O2损伤新西兰白兔颈总动脉制备血栓模型，造模2 h后，采血并取血栓，酶联免疫吸附法(ELISA法)检测血浆tPA、PAI1的含量。结果 与空白组比较，模型组tPA明显降低，PAI1明显升高，差异有统计学意义(P<0.01)；与模型组相比，全蝎纯化液各剂量组能明显抑制PAI1活性，增加tPA活性，差异均有统计学意义(P<0.01),而各组的血小板计数差异无统计学意义(P>0.05)。结论 全蝎纯化液各剂量组可明显促进血浆tPA的分泌，抑制血浆PAI1活性，提示全蝎纯化液抗血栓作用机制可能与其促纤溶作用有关。
关键词：全蝎；新西兰白兔；动脉血栓；组织型纤溶酶原激活物；纤溶酶原活化物抑制剂
中图分类号：R543.5 R285.5 文献标识码：A 文章编号：16721349(2012)02019502
The Influence of Scorpion Purified Liquid on tPA and PAI1 of Experimental Arterial Thrombosis in Rabbit
Zhao Jianying,Shi Diao,Tian Qian,et alVascular Biology Laboratory,Hunan University of Chinese Medicine (Changsha 410007)
Abstract:Objective To study the effect of scorpion purified liquid on tissuetype plasminogen activator (tPA) and plasminogen activator inhibitor1 (PAI1) in plasma of carotid arterial thrombosis of New Zealand white rabbit.Methods Fifty healthy New Zealand white rabbits were randomly assigned into five groups:Blank group,model group,scorpion purified liquid high dose (20 mg/kg),scorpion purified liquid medium dose (10 mg/kg),and scorpion purified liquid low dose (5 mg/kg).Scorpion purified liquid and equal volume of saline were injected via ear vein.The carotid arterial thrombosis rabbit models were prepared by H2O2 injection.Plasma tPA and PAI1 were detected after two hours of model preparation.Results The levels of plasma PAI1 were decreased while that of plasma tPA were increased in model group (P<0.01).There was no significant differences in platelet between model group and scorpion purified liquid groups(P>0.05).Conclusion Scorpion purified liquid could upregulate the level of plasma tPA and inhibit activity of plasma PAI1 in carotid arterial thrombosis of rabbit.Its mechanism may be related to anticoagulation and promote fibrinolysis.
Key words:scorpion;New Zealand white rabbits;arterial thrombosis;tissuetype plasminogen activator;plasminogen activator inhibitor1
心脑血管疾病已成为人类健康的最大威胁之一[1] ......
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