Effctof Phloretinon Rats lschemia-reperfusion Myocardial Injuryby Regulating the Shh/Gli1 SignalingPathway ZHANG Zhiling，DI Xueqin，MA Xiaoru，MA Shuyi，LIFangfang

    BaodingNo.1Central Hospital，BaodingO72150，Hebei，China Corresponding Author Dl Xueqin，E-mail： 149939389@ qq.com

    AbstractObjective：ToexploretheefectofphloretinPhtonratsischemia-eperfusionmyocardialijury(Ml)byregulatingthesonic hedgehog/gloaoatedcogene1(SGli)signaigpatayMethds：Atotalof90ealtyaleSDatsofspeificptof (SPF)grade wererandomlydividedintothecontrol group(Control group)，themodel group(MIRlgroup)，thelow-dosephytoltreatment group(Pht-L)，thehighdosephytoltreatmentgroup(Pht-H)，andtheigh-dosephytoltreatment+cyclopamine(aGliShhsignaling pathwayinhibitorgroup(PhtHCycgroup).TheMIRIratmodelwasestablishedbyligatingtheleftanteriordescendingbranchofthe coronaryarterysfitoctoossfctteri-ββ)dd indicatorssuchascreatinekinaseisoenzyme(CK-MB)，troponinI(cTnl)，andmyoglobin(Mb)weredetectedbyenzymeinked immunosorbentassay(ELSA).Hematoxylineosin(HE)stainingwasusedtoobservethemorphologicalchangesofmyocardiatissue. terminaldeoxibonucleotidyltransferase(TdTmediateddUTPnickendlabelingUNEL)stainingwasusedtodetect theapoptosisof cardiacmusclecelexpessonveofcelo-cl-2)c-soatedproteiax)ndGetedy WesterBlotResults：ComparedwiththeControlgroup ......