黏蛋白Muc5ac在大鼠慢性支气管炎\肺气肿模型肺部的表达(1)
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[摘要] 目的:探讨香烟对大鼠慢性支气管炎、肺气肿模型的诱导及呼吸道上皮细胞黏液蛋白Muc5ac表达的影响。方法:采用熏香烟法制造大鼠慢性支气管炎、肺气肿模型,采用免疫组化法测定模型肺组织中黏蛋白Muc5ac的表达。结果:熏香烟组大鼠肺体积较对照组大鼠增大,颜色较苍白,部分肺表面可见多个大小不等的过度充气肺大泡,肺弹性减弱;光镜下纤毛脱落、倒伏,支气管黏膜上皮坏死、脱落,有炎症细胞浸润,肺泡大小不等、肺泡壁变薄,并有不同程度的断裂,部分融合成肺大泡;免疫组化染色结果显示实验组大鼠肺内支气管上皮细胞、肺泡上皮细胞Muc5ac表达与对照组比较明显增强(P<0.001)。结论:熏香烟法可成功诱导大鼠发生慢性支气管炎合并肺气肿模型,并导致肺组织黏蛋白Muc5ac的明显表达。
[关键词] Muc5ac蛋白;慢性支气管炎;肺气肿;烟
[Abstract] Objective: The aim of current study is to establish a rat model of chronic bronchitis and emphysema induced by cigarette smoking(CS) and evaluate the effect of expression of mucin Muc5ac producted by airway epithelial cells.Methods: Establish rat models of chronic bronchitis and emphysema by cigarette smoking, and detect the expression of mucin Muc5ac in model lung with immunocytochemical techniques ......
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