分化型甲状腺癌肿瘤局部免疫细胞亚群与其复发关系(1)
[摘要] 目的 研究分化型甲狀腺癌(DTC)免疫细胞亚群局部浸润相关模式，探讨肿瘤局部免疫细胞亚群与DTC复发的相关性。方法 TCGA数据库下载DTC病人转录本及临床相关数据，应用CIBERSORT软件反卷积算法计算22种免疫细胞在DTC病人癌组织与癌旁组织中所占比例，Kaplan-Meier法绘制生存曲线，Log-rank检验分析DTC病人肿瘤复发与免疫细胞亚群的关系。结果 TCGA数据库中下载509例DTC样本，根据病人无病生存期数据应用CIBERSORT软件以P<0.05为条件筛选出匹配样本184例，其中癌旁组织17例，癌组织167例。Kaplan-Meier生存分析显示，NK细胞高比例DTC病人1年无病生存率为98.7%，5年无病生存率为93.6%;而NK细胞低比例DTC病人1年无病生存率为92.0%，5年无病生存率为65.5%，两组5年无病生存率差异有显著性(χ2=7.591，P<0.05)。结论 DTC免疫细胞亚群与临床结果密切相关，活化的NK细胞比例增加可提高甲状腺癌病人无病生存率，针对NK细胞的免疫疗法可能成为预防DTC复发的选择之一。
[关键词] 甲状腺肿瘤;肿瘤复发，局部;免疫细胞
[中图分类号] R736.1;R73-37 [文献标志码] A [文章编号] 2096-5532(2020)05-0566-05
doi：10.11712/jms.2096-5532.2020.56.084 [开放科学(资源服务)标识码(OSID)]
[ABSTRACT] Objective To investigate the local infiltration pattern of immune cell subsets in differentiated thyroid carcinoma (DTC) and the relationship between DTC recurrence and subsets of tumor-infiltrating immune cells. Methods TCGA database was used to download the transcripts and clinical data of patients with DTC. The deconvolution algorithm in CIBERSORT software was used to calculate the proportion of 22 types of infiltrating immune cells in cancerous tissues and adjacent tissues of DTC patients. The Kaplan-Meier method was adopted to plot survival curves. The Log-rank test was used to analyze the relationship between tumor recurrence and infiltrating immune cell subsets in DTC patients. Results A total of 184 matched samples were screened out of 509 cases of DTC samples in the TCGA dataset using the CIBERSORT software according to the patients’ disease-free survival data under the condition of P<0.05， including 17 cases of adjacent tissues and 167 cases of cancerous tissues. The Kaplan-Meier survival analysis showed that for DTC patients with high NK cell proportion， the 1 year and 5 year disease-free survival rates were 98.7% and 93.6%， respectively， but for DTC patients with low NK cell proportion， the rates were 92.0% and 65.5%， respectively. There was a significant difference in 5 year disease-free survival rate between the two groups (χ2=7.591，P<0.05). Conclusion The subsets of tumor-infiltrating immune cells in DTC patients are closely related to the clinical outcome. Increased proportion of activated NK cells can significantly reduce DTC recurrence rate. In the future， immunotherapy based on NK cells may become one of the options for preventing the recurrence of DTC.
[KEY WORDS] thyroid neoplasms; neoplasm recurrence， local; immune cells, 百拇医药(王翔 尹雅楠 孔姿莉 王颜刚)
[关键词] 甲状腺肿瘤;肿瘤复发，局部;免疫细胞
[中图分类号] R736.1;R73-37 [文献标志码] A [文章编号] 2096-5532(2020)05-0566-05
doi：10.11712/jms.2096-5532.2020.56.084 [开放科学(资源服务)标识码(OSID)]
[ABSTRACT] Objective To investigate the local infiltration pattern of immune cell subsets in differentiated thyroid carcinoma (DTC) and the relationship between DTC recurrence and subsets of tumor-infiltrating immune cells. Methods TCGA database was used to download the transcripts and clinical data of patients with DTC. The deconvolution algorithm in CIBERSORT software was used to calculate the proportion of 22 types of infiltrating immune cells in cancerous tissues and adjacent tissues of DTC patients. The Kaplan-Meier method was adopted to plot survival curves. The Log-rank test was used to analyze the relationship between tumor recurrence and infiltrating immune cell subsets in DTC patients. Results A total of 184 matched samples were screened out of 509 cases of DTC samples in the TCGA dataset using the CIBERSORT software according to the patients’ disease-free survival data under the condition of P<0.05， including 17 cases of adjacent tissues and 167 cases of cancerous tissues. The Kaplan-Meier survival analysis showed that for DTC patients with high NK cell proportion， the 1 year and 5 year disease-free survival rates were 98.7% and 93.6%， respectively， but for DTC patients with low NK cell proportion， the rates were 92.0% and 65.5%， respectively. There was a significant difference in 5 year disease-free survival rate between the two groups (χ2=7.591，P<0.05). Conclusion The subsets of tumor-infiltrating immune cells in DTC patients are closely related to the clinical outcome. Increased proportion of activated NK cells can significantly reduce DTC recurrence rate. In the future， immunotherapy based on NK cells may become one of the options for preventing the recurrence of DTC.
[KEY WORDS] thyroid neoplasms; neoplasm recurrence， local; immune cells, 百拇医药(王翔 尹雅楠 孔姿莉 王颜刚)