HPLC法测定垂盆草提取物中异鼠李素的含量
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[摘要] 目的：用HPLC法测定垂盆草提取物中异鼠李素的含量。方法：采用ODS-C18(150 mm×4.6 mm，5 μm)色谱柱，流动相为甲醇-0.4%磷酸(55︰45)；流速1.0 ml/min；检测波长360nm。结果：异鼠李素浓度在2.82~28.20 μg/ml范围内线性良好，r=0.999 8(n=8)，平均回收率为100.18%，RSD为1.73%(n=9)。结论：本法可用于控制垂盆草中有效成分的含量，简便、准确、灵敏度高。
[关键词] 垂盆草；提取物；含量测定；HPLC
[中图分类号]R917[文献标识码]B[文章编号] 1673-7210(2007)12(c)-160-02
Content determination of isorhamnetin in the extract of Sedum sarmentosum Bunge by HPLC
MA Jia-lei, LI Qun-li*, XU Ling-ling
(Medical College of Jinhua Profession and Technology College, Jinhua321007, China)
[Abstract] Objective: To develop a method for the content determination of isorhamnetin in Sedum sarmentosum Bunge. Methods: The samples were analyzed on an ODS-C18 column with mobile phase of methanol-0.4% phosphoric acid (55︰45) at flow rate 1.0 ml/min and detective wavelength of 360 nm. Results: The standard curves were linear relationship at the range of 2 ......
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