薄层层析-紫外分光光度法测定丹参注射液中原儿茶醛的含量
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[摘要] 目的：建立薄层层析-紫外分光光度法测定丹参注射液中原儿茶醛的含量。方法：以原儿茶醛为对照品，二氯甲烷-乙酸乙酯-甲酸(8∶5∶0.8)为展开剂，分离得到原儿茶醛，采用紫外分光光度法，检测波长281 nm，测定原儿茶醛的含量。结果：原儿茶醛在0.50~4.50 μg/ml 范围内线性关系良好，r=0.999 7。平均含量为0.202 mg/ml，加样回收率为98.0%~103.0%。结论：实验结果表明，薄层层析-紫外分光光度法测定丹参注射液中原儿茶醛的含量，方法操作简便、准确度高、精密度好，可用于丹参注射液的质量控制。
[关键词] 丹参注射液；原儿茶醛；薄层层析-紫外分光光度法；含量测定
[中图分类号]R917 [文献标识码]A [文章编号]1673-7210(2008)01(b)-050-02
Content determination of protocatechuic aldehyde in Danshen Injection by TLC-UV method
LI Long-jian, LU Jin-hua
(Chinese Armed Police Forces Zhejiang Province Corps Hospital, Jiaxing 314000, China)
[Abstract] Objective: To separate and determine the content of protocatechuic aldehyde in Danshen Injection by TLC-UV method. Methods: Using protocatechuic aldehyde as reference substance, the content of protocatechuic aldehyde in Danshen Injection was determined by TLC-UV with dichloromethane-acetic ether- acetic ether(8∶5∶0.8) as the developing solvent and the detective wavelength of 281 nm.Results: Protocatechuic aldehyde had a good linearity in the range of 0 ......
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