苦玄参不同提取部位抑制2215细胞分泌HBeAg和HBsAg的实验研究(1)
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[摘要] 目的:观察苦玄参不同提取部位的含药血清对2215细胞分泌HBeAg和HBsAg的抑制作用。方法:将含药血清作用于2215细胞,然后用酶联免疫法测定HBsAg和HBeAg的水平。结果:苦玄参各提取部位对HBeAg有明显的抑制作用,但没有明显的量效关系。其中乙醇总提取部位、水提取部位和正丁醇提取部位对HBeAg的抑制效果比乙酸乙酯提取部位好,抑制率最高可达80%以上(P<0.05);苦玄参各提取部位对HBsAg也有一定的抑制作用,不同提取部位的抑制作用相差不大。结论:苦玄参不同提取部位的含药血清能抑制2215细胞分泌HBeAg和HBsAg,其中极性较大的化合物对HBeAg的抑制作用较强。
[关键词] 苦玄参;2215细胞;酶联免疫法;血清药理学
[中图分类号] R329.2+8[文献标识码]A [文章编号]1673-7210(2010)06(a)-027-03
Experimental study on the inhibitory effect on HBeAg and HBsAg excreted by 2215 cells of different extracts of Picria fel-tarrae Lour
ZENG Jinqiang1*, PAN Xiaojiao2 ,YANG Ke2, WEI Zhiying2, CHEN Chen2
(1.Guangxi Frontier Hospital, Nanning 530022, China; 2.Guangxi Traditional Chinese Medical University, Nanning 530001, China)
[Abstract] Objective: To observe the inhibitory effect on HBeAg and HBsAg excreted by 2215 cells of different extracts in serums of Picria fel-tarrae Lour. Methods: The different extracts in serums of Picria fel-tarrae Lour were used to act on 2215 cells and the enzyme linked immune response was used to investigate the level of HBeAg and HBsAg. Results: All extracts showed the obvious inhibitory activity on HBeAg, but there wasn't any obvious dose-effect relationship. The EtOH, BuOH and H2O extracts showed the better inhibitory activity on HBeAg than the EtOAc extracts did. The best inhibitory rates of them on HBeAg were more than 80% (P<0.05). All extracts showed some inhibitory activity on HBsAg and this kind of activity was similar among them. Conclusion: The different extracts of Picria fel-tarrae Lour in serums have the inhibitory effect on HBeAg and HBsAg excreted by 2215 cells and the compounds possessing of bigger polarity show the stronger inhibitory activity.
[Key words] Picria fel-tarrae Lour; 2215 cells; Enzyme linked immune response; Seropharmacology
苦玄参为玄参科植物苦玄参(Picria fel-tarrae Lour)的干燥全草,别名苦草、蛇总管。苦玄参味苦、性寒,具有清热解毒、消肿止痛的功能,用于感冒风热、咽喉肿痛、痄腮、胃热腹痛、痢疾、跌打损伤、疖痈、毒蛇咬伤等[1]。苦玄参在广西壮族自治区已得到较好的开发应用,多个以苦玄参为主要成分的中成药已上市销售[2]。目前对苦玄参只有解热和抗菌的药理研究[3-4],尚无其抗乙肝的相关试验研究报道。笔者对苦玄参不同提取部位含药血清的体外抗乙肝作用进行了初步研究,现报道如下:
1 材料与方法
1.1 药材
苦玄参经广西中医学院药用植物教研室韦松基副教授鉴定为玄参科植物苦玄参(Picria fel-tarrae Lour.)的干燥全草。
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