类风湿关节炎患者外周血白细胞介素-23和类风湿因子的表达研究(1)
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[摘要] 目的：检测类风湿关节炎(RA)患者体内白细胞介素(IL)-23和类风湿因子(RF)的表达，探讨IL-23在RA发病中的作用及其与RF的关系，为RA的早期诊断和进一步阐明RA的发病机制提供理论依据。方法：采用逆转录-聚合酶链反应(RT-PCR)检测66例RA患者和44例健康对照者外周血单个核细胞(PBMC)中IL-23p19 mRNA的表达水平，采用酶联免疫吸附试验(ELISA)检测血清IL-23浓度，采用免疫比浊法检测血清RF的浓度。结果：RT-PCR显示RA患者PBMC中IL-23p19 mRNA的表达[(45.2±11.7)%]显著高于健康对照组[(23.8±9.5)%]，ELISA检测结果显示RA患者血清IL-23水平[(114.47±42.70)ng/L]显著高于健康对照组[(70.81±28.85)ng/L]，差异均有统计学意义(均P<0.05)。RA患者血清IL-23水平与RF含量无明显相关性(r=0.389，P>0.05)。结论：RA患者体内IL-23在基因和蛋白水平均过表达，提示IL-23可能参与了RA的免疫病理过程，在RA的发病中发挥作用。
[关键词] 类风湿关节炎；白细胞介素-23；类风湿因子
[中图分类号] R593.22 [文献标识码] A [文章编号] 1673-7210(2011)06(c)-023-03
The study of interleukin-23 and rheumatoid factor expression in peripheral blood of patients with rheumatoid arthritis
ZHNAG Guohua1,2, LU Zhi1*
1.Second Clinical Medical College, Shanxi Medical University, Shanxi Province, Taiyuan 030001, China; 2.Fenyang College of Shanxi Medical University, Shanxi Province, Fenyang 032200, China
[Abstract] Objective: To detect the expression of interleukin (IL)-23 and rheumatoid factor (RF) in rheumatoid arthritis (RA) patients and to explore the role of IL-23 in the pathogenesis of RA and its relationship with the RF, for early diagnosis of RA and further clarify the pathogenesis of RA provides theoretical basis. Methods: IL-23p19 mRNA expression in peripheral blood mononuclear cells (PBMCs) of 66 RA patients and 44 healthy controls was detected by reverse transcription-polymerase chain reaction (RT-PCR), serum IL-23 concentration were measured by enzyme-linked immunosorbent assay (ELISA), serum RF concentrations were measured by immunoturbidimetry. Results: RT-PCR showed IL-23p19 mRNA expression in PBMC of RA patients [(45.2±11.7)%] was significantly higher than that of healthy controls [(23.8±9.5)%], ELISA results showed IL-23 concentration in serum of RA patients [(114.47±42.70) ng/L] was significantly higher than that in healthy controls [(70 ......
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