七氟烷后处理对大鼠在体心肌抗氧化的影响(1)
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[摘要] 目的：探讨七氟烷后处理对大鼠在体心肌抗氧化的作用及最佳浓度。方法：将40只健康雄性SD大鼠分为5组，即假手术组(C组)、缺血再灌注组(IP组)、1%、2%、3%七氟烷后处理组(S1、S2、S3组)，每组各8只，建立大鼠在体心肌缺血再灌注模型。测定再灌注3 h后血浆MDA、SOD和CK-MB含量和心肌梗死面积。结果：与IP组相比，各S组心肌梗死面积、MDA、CK-MB均显著降低(P<0.05)，SOD含量均显著升高(P<0.05)。S1组与S3组各指标间比较，差异无统计学意义(P>0.05)。与S1、S3组相比，S2组心肌梗死面积(28.5±1.35)%、MDA(8.205±2.874) nmol/ml、CK-MB(22.935±2.064) U/L均显著降低(P<0.05)，SOD含量显著升高(P<0.05)。结论：七氟烷后处理可通过调节MDA与SOD的表达，发挥心肌保护作用，且中剂量组(2%七氟烷)效果更明显。
[关键词] 七氟烷后处理；心肌缺血再灌注；抗氧化
[中图分类号] R965 [文献标识码]A [文章编号]1673-7210(2011)07(b)-015-02
The effect of sevoflurane postconditioning on antioxidation of rat hearts in vivo
LI Jing, HAN Chongfang, LI Xiaoxin, DU Yi, YANG Wenqu
Department of Anesthesiology, the First Affiliated Hospital of Shanxi Medical University, Shanxi Province, Taiyuan 030001, China
[Abstract] Objective: To investigate the antioxidation of sevoflurane postconditioning in rat hearts in vivo and the best concentration. Methods: A total of 40 SD rats were randomly divided into 5 groups, sham operation group (group C), ischemia and reperfusion group (group IP), 1%, 2%, 3% sevoflurane postconditioning group (group S1, group S2, group S3), 8 in every group, the model of rats′ myocardial ischemia reperfusion was established. After being reperfused for 3 h, the blood SOD, MDA, CK-MB and myocardial infarct size were measured. Results: Compared with group IP, expression of myocardial infarct size, MDA, CK-MB in S groups were significantly lower (P<0.05), while the level of SOD was significantly higher (P<0.05). There was no significant difference between group S1 and group S3 (P>0.05). Compared with group S1 and group S3, expression of myocardial infarct size (28.5±1.35)%, MDA (8.205±2.874) nmol/ml, CK-MB (22.935±2.064) U/L in group S2 were significantly lower (P<0.05), while the level of SOD was significantly higher (P<0.05). Conclusion: Postconditioning with sevoflurane protects the heart of the rat against ischemia reperfusion injury, and 2% sevoflurane has a better protective effect.
[Key words] Sevoflurane postconditioning; Myocardial ischemia reperfusion; Antioxidation ......
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