基于反相液相色谱的人血中茶碱浓度临床检测与分析(1)
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[摘要] 目的：建立快速、简便、准确、重现性好的人血中茶碱的测定方法。方法：采用Hypersil ODS C18(4.6 mm×250 mm，5 μm)为色谱柱，流动相为甲醇-水(25∶75)，内标物为咖啡因，检测波长为274 nm，血清用甲醇沉淀蛋白后进行分析。结果：茶碱标准曲线回归方程为Y=0.053 9X+0.038，r=0.999 4(n=8)，1～40 μg/ml浓度范围线性关系良好。对36例患者作血清茶碱浓度监测，临床效果良好。结论：本测定方法为临床药师指导临床有效、安全的个体化药物的使用提供了保障。
[关键词] 茶碱；血药浓度；RP-HPLC；个体化用药
[中图分类号] R927.2 [文献标识码] A [文章编号] 1673-7210(2011)12(a)-074-03
Detection of theophylline in human serum and its clinical application based on reversed-phase HPLC
DUN Wenliang1, ZHOU Yu2, GAO Yunjun1, Bao Beihua2
1.Department of Pharmacy, Nanjing Hospital of Traditional Chinese Medicine, Jiangsu Province, Nanjing 210001, China; 2.College of Pharmaceutical, Nanjing University of Chinese Medicine, Jiangsu Province, Nanjing 210029, China
[Abstract] Objective: To develop a rapid, simple, accurate and reproducible method for the determination of theophylline in human serum. Methods: The separation was performed on Hypersil ODS C18 column (4.6 mm×250 mm, 5 μm) with mobile phase consisted of methanol-water(25∶75), and the wavelength for detection was 274 nm. The coffeine was used as an internal standard, and methanol was used to precipitate the serum protein. Results: The regression equation of theophylline was Y=0.053 9X+0.038, r=0.999 4 (n=8). The calibration curves of theophylline were linear over the range of 1-40 μg/ml. The clinical application was good for 36 patients through of the detection of theophylline. Conclusion: The method supplies a reasonable guide for the drug use at individual levels.
[Key words] Theophylline; Serum concentration; Reversed-phase HPLC; Individualized medication
茶碱是临床上最常用的平喘药之一，但茶碱的体内吸收和排泄个体差异很大，并且其有效范围窄，易产生副作用，因此监测茶碱血药浓度对临床治疗显得非常必要[1]。目前HPLC是最常用方法之一，满足临床需要，本实验结合文献[2]，建立了快速、准确、简便、经济、重现性好的茶碱血药浓度的HPLC检测方法。
1 仪器与试药
1.1 仪器
日本岛津LC-10Avp高效液相色谱仪，LC-10ADVP双泵，SPD-10Avp紫外检测器，LC solution色谱工作站，Ts 0.5/20型超声波电源，HP-01真空泵，1612-高速离心机，SK-1型快速混匀器，SC-230海尔冰箱，AL104电子天平。
1.2 药品与试剂
茶碱(购自中国药品生物制品检定所，批号：100121-199903)、咖啡因(购自中国药品生物制品检定所，批号：171215-200809，含量为99.9%)，甲醇为色谱纯 ......
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