骨基质明胶在异位成骨区的成骨研究(1)
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[摘要] 目的 探讨不同处理后的骨基质明胶(BMG)在异位成骨区的成骨效果。 方法 将36只日本大耳白兔随机分为3组，每组12只。A组血管束植入用bFGF预处理过的BMG中；B组血管束植入未作任何预处理的BMG中；C组单独植入BMG。分别于术后4、8、12周给各组动物灌注墨汁后做HE及Masson染色进行组织学观察和定量分析。 结果 新生骨面积定量分析，各个时间点上A组新生骨面积均最大，与C组比较差异均有统计学意义(均P ﹤ 0.05)。新生骨面积比与血管面积比相关分析结果显示，r4周=0.53，P﹤0.05；r8周=0.46，P﹤0.05；r12周=0.61，P﹤0.05。 结论 血管化程度越高，新生骨面积越大；新生骨面积比与血管化程度呈正相关。
[关键词] 骨基质明胶；血管化；碱性成纤维细胞生长因子
[中图分类号] R318.17 [文献标识码] A [文章编号] 1673-7210(2012)02(a)-0019-03
The osteogenic effect of bone matrix gelatin in ectopic bone area femoral
WANG Ruifang1 QIN Shujian2 TIAN Zhifeng3
1.Department of Anatomy, Shanxi Medical University Fenyang College, Shanxi Province, Fenyang 032200, China; 2.Liaoning Medical University, Liaoning Province, Jinzhou 121001, China; 3.Department of Anatomy, Luohe Medical College, He'nan Province, Luohe 462002, China
[Abstract] Objective To investigate the osteogenic effect of bone matrix gelatin after different dealing in ectopic bone area femoral. Methods 36 Japanese big ears white rabbits were divided into group A, B and C (12 in each). Group A: bFGF+Vascular bundle+BMG. Group B: Vascular bundle+BMG. Group C: BMG. The animals were sacrificed after 4, 8 and 12 weeks respectively by perfusing ink. HE and Masson stained for histological observations and quantitative analysis. Results Quantitative analysis showed that the osteogenic and neovascularization areas of group A were the largest on the every time point. The correlation analysis of osteogenic area and vascular area were r4 weeks=0.53, P ﹤ 0.05; r8 weeks=0.46, P﹤0.05; r12 weeks=0.61, P﹤0.05. Conclusion The better vascularization, the better osteogenesis. There is a positive correlation between osteogenesis and vascularization.
[Key words] Bone matrix gelatin; Vascularization; Basic fibroblast growth factor
骨折愈合是一个受多因素影响的复杂过程，其中骨折区的血液供应对骨折愈合的效果起到非常重要的作用。本实验旨在研究不同处理后的骨基质明胶(bone matrix gelatin，BMG)在兔股前内侧区的成骨效果，为临床骨折修复提供实验依据。
1 材料与方法
1.1 实验动物
健康5月龄日本大耳白兔40只，体重1.5～2.0 kg，雌雄不限。
1.2 制备BMG
取4只日本大耳白兔四肢骨、髂骨，将四肢骨的骺端及髂骨参照Urist[1]的方法制备成6 mm×5 mm×4 mm大小的BMG。
1.3 BMG的植入
将36只日本大耳白兔随机分为3组，10%水合氯醛腹腔麻醉后手术，在膝关节上方的股内侧区做一纵行切口，A组：BMG用2 μg/mL碱性成纤维细胞生长因子(Basic fibroblast growth factor，bFGF)浸泡过后将隐动、静脉游离1 cm后顺行植入；B组：将隐动、静脉游离1 cm后植入未作任何预处理的BMG中；C组则直接将未处理过的BMG植入 ......
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