P53蛋白在胃癌中的表达分析(1)
[摘要] 目的 探讨P53蛋白在胃癌中的表达。 方法 胃癌患者53例作为A组，胃炎患者53例作为B组，健康人士53例作为C组，均采用免疫组织化学法(S-P)检测P53阳性率。 结果 A组P53阳性率为64.2%，B组为32.1%，C组为13.2%，组间比较差异有统计学意义(A、B：χ2=9.674，P <0.01；A、C：χ2=26.888，P <0.01；B、C：χ2=4.363，P <0.05)。A组高分化癌P53阳性率为78.6%，低分化癌为48.0%，两者比较差异有统计学意义(χ2=4.120，P <0.05)。B组浅表性胃炎P53阳性率为40.0%，萎缩性胃炎为21.7%，萎缩性胃炎P53阳性率高于浅表性胃炎，但差异无统计学意义(χ2=1.243，P >0.05)。 结论 对胃病患者进行P53的早期检测，对监测病情和改善预后具有重要意义，值得临床考虑。
[关键词] P53；胃癌；表达
[中图分类号] R735.2 [文献标识码] A [文章编号] 1673-7210(2012)02(b)-0086-02
, http://www.100md.com
Analysis on expression of positive rate of P53 protein in patients with gastric cancer
HE Junhui1 ZOU Xiuling2 LI Zhongchu3 LU Zhongkui4
1.The Third Department of Medical, Daliang Hospital of Shunde District in Foshan City, Guangdong Province, Foshan 528300, China; 2.Department of Oncology, Longjiang Hospital of Shunde District in Foshan City, Guangdong Province, Foshan 528318, China; 3.The Third Department of Medical, Longjiang Hospital in Shunde District in Foshan City, Guangdong Province, Foshan 528318, China; 4. Department of Laboratory, Longjiang Hospital of Shunde District in Foshan City, Guangdong Province, Foshan 528318, China
, 百拇医药
[Abstract] Objective To study the expression of positive rate of P53 protein in patients with gastric cancer. Methods 53 cases of patients with gastric cancer as group A, 53 cases of patients with gastritis as group B and 53 cases of healthy person as group C were all given detection of positive rate of P53 by S-P method. Results The positive rate of P53 in group A was 78.6%; group B was 48.0%; group C was 13.2%. There were significant difference's between each group (A, B: χ2=9.674, P <0.01；A, C: χ2=26.888, P <0.01; B, C: χ2=4.363, P <0.05). The positive rate of P53 in group A of differentiated carcinoma was 78.6%; poor differentiated carcinoma was 48.0%. The comparison between the two showed significant difference (χ2=4.120, P <0.05) . The positive rate of P53 in group B of superficial gastritis was 40.0%; atrophic gastritis was 21.7%. The superficial gastritis was higher, but no significant difference (χ2=1.243，P >0.05). Conclusion Detection of P53 of patients with gastropathy at early stage is very important on monitoring disease and changing prognosis, so it deserves considering in clinical work.
, 百拇医药
[Key words] P53; Gastric cancer; Expression
胃癌是我国常见多发恶性肿瘤疾病之一。胃癌的发生和进展受到多种因子的调控和影响，从基因水平对其发病机制进行研究，有助于胃癌高危患者的大规模筛查，提高胃癌诊断率，并在治疗过程中提供相对客观的观察指标。目前关于相关因子的研究较多，如Mdm2基因、P53基因、Cyclin G1基因和HP相关基因等，其中P53是研究较深入和较早的抑癌基因，而且上述各基因作为P53的下游产物，其表达和P53表现出不同程度的蛋白依赖性。因此对P53基因展开研究，有助于更为基础的研究其发病机制。笔者对健康人群、胃炎患者和胃癌患者分别展开P53蛋白表达率的检测，现报道如下：
1 资料与方法
1.1 一般资料
选择2011年3~8月前来我院接受治疗的胃癌患者作为A组，共53例，其中高中分化腺癌28例，低分化腺癌25例；男31例，女22例；年龄38~74岁，平均56.3岁。选择胃炎患者作为B组，共53例，其中浅表性胃炎30例，萎缩性胃炎23例；男32例，女21例；年龄39~73岁，平均56.7岁。选择健康体检患者作为C组，共53例，男33例，女22例；年龄39~72岁，平均56.4岁。三组在年龄、性别方面比较差异无统计学意义(P >0.05)，具有可比性。
1.2 方法
收集三组患者胃黏膜组织块标本，均采用10%多聚甲醛溶液固定，常规石蜡包埋，做厚度为5 μm的均匀切片，进行免疫组织化学染色和常规HE染色。免疫组化S-P试剂盒和P53单克隆抗体均购自北京中山生物技术有限公司。采用免疫组织化学法(S-P)检测P53。每例切片观察10个高倍视野，阳性表达产物为棕黄色颗粒，记数1 000个癌细胞中阳性细胞所占百分率，百分率≥10%为阳性，百分率<10%为阴性。, http://www.100md.com(何均辉 邹秀玲 李忠楚 陆忠奎)
[关键词] P53；胃癌；表达
[中图分类号] R735.2 [文献标识码] A [文章编号] 1673-7210(2012)02(b)-0086-02
, http://www.100md.com
Analysis on expression of positive rate of P53 protein in patients with gastric cancer
HE Junhui1 ZOU Xiuling2 LI Zhongchu3 LU Zhongkui4
1.The Third Department of Medical, Daliang Hospital of Shunde District in Foshan City, Guangdong Province, Foshan 528300, China; 2.Department of Oncology, Longjiang Hospital of Shunde District in Foshan City, Guangdong Province, Foshan 528318, China; 3.The Third Department of Medical, Longjiang Hospital in Shunde District in Foshan City, Guangdong Province, Foshan 528318, China; 4. Department of Laboratory, Longjiang Hospital of Shunde District in Foshan City, Guangdong Province, Foshan 528318, China
, 百拇医药
[Abstract] Objective To study the expression of positive rate of P53 protein in patients with gastric cancer. Methods 53 cases of patients with gastric cancer as group A, 53 cases of patients with gastritis as group B and 53 cases of healthy person as group C were all given detection of positive rate of P53 by S-P method. Results The positive rate of P53 in group A was 78.6%; group B was 48.0%; group C was 13.2%. There were significant difference's between each group (A, B: χ2=9.674, P <0.01；A, C: χ2=26.888, P <0.01; B, C: χ2=4.363, P <0.05). The positive rate of P53 in group A of differentiated carcinoma was 78.6%; poor differentiated carcinoma was 48.0%. The comparison between the two showed significant difference (χ2=4.120, P <0.05) . The positive rate of P53 in group B of superficial gastritis was 40.0%; atrophic gastritis was 21.7%. The superficial gastritis was higher, but no significant difference (χ2=1.243，P >0.05). Conclusion Detection of P53 of patients with gastropathy at early stage is very important on monitoring disease and changing prognosis, so it deserves considering in clinical work.
, 百拇医药
[Key words] P53; Gastric cancer; Expression
胃癌是我国常见多发恶性肿瘤疾病之一。胃癌的发生和进展受到多种因子的调控和影响，从基因水平对其发病机制进行研究，有助于胃癌高危患者的大规模筛查，提高胃癌诊断率，并在治疗过程中提供相对客观的观察指标。目前关于相关因子的研究较多，如Mdm2基因、P53基因、Cyclin G1基因和HP相关基因等，其中P53是研究较深入和较早的抑癌基因，而且上述各基因作为P53的下游产物，其表达和P53表现出不同程度的蛋白依赖性。因此对P53基因展开研究，有助于更为基础的研究其发病机制。笔者对健康人群、胃炎患者和胃癌患者分别展开P53蛋白表达率的检测，现报道如下：
1 资料与方法
1.1 一般资料
选择2011年3~8月前来我院接受治疗的胃癌患者作为A组，共53例，其中高中分化腺癌28例，低分化腺癌25例；男31例，女22例；年龄38~74岁，平均56.3岁。选择胃炎患者作为B组，共53例，其中浅表性胃炎30例，萎缩性胃炎23例；男32例，女21例；年龄39~73岁，平均56.7岁。选择健康体检患者作为C组，共53例，男33例，女22例；年龄39~72岁，平均56.4岁。三组在年龄、性别方面比较差异无统计学意义(P >0.05)，具有可比性。
1.2 方法
收集三组患者胃黏膜组织块标本，均采用10%多聚甲醛溶液固定，常规石蜡包埋，做厚度为5 μm的均匀切片，进行免疫组织化学染色和常规HE染色。免疫组化S-P试剂盒和P53单克隆抗体均购自北京中山生物技术有限公司。采用免疫组织化学法(S-P)检测P53。每例切片观察10个高倍视野，阳性表达产物为棕黄色颗粒，记数1 000个癌细胞中阳性细胞所占百分率，百分率≥10%为阳性，百分率<10%为阴性。, http://www.100md.com(何均辉 邹秀玲 李忠楚 陆忠奎)