紫外-可见分光光度法测定普乐安片中总甾醇含量(1)
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[摘要] 目的 建立紫外-可见分光光度法测定普乐安片中总甾醇的含量测定方法。 方法 以β-谷甾醇为对照品，通过香草醛-高氯酸显色反应，测定普乐安片中总甾醇的含量。 结果 对照品和样品最大吸收波长均在542 nm，对照品β-谷甾醇在0.030 39～0.081 04 mg范围内线性关系良好，r = 0.998 8，样品平均回收率为100.6%，RSD=2.75%。 结论 本法测定总甾醇含量准确、灵敏，重现性好，可用于测定普乐安片中总甾醇含量，为普乐安片质量控制提供可靠依据。
[关键词] 紫外-可见分光光度法；β-谷甾醇；普乐安片；总甾醇；含量测定
[中图分类号] R285.5 [文献标识码] A [文章编号] 1673-7210(2012)08(b)-0008-03
Quantitative determination of total sterol in Pule'an Tablets by UV spectrophotometry
SHENG Weiguo YE Jianfeng XU Yong WU Jian MA Liping WANG Ruwei
Zhejiang Modern Chinese Medicine and Natural Drug Research Academy Co., LTD. Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmacecutical Technology, Zhejiang Province, Hangzhou 310052, China
[Abstract] Objective To establish the determination method of total sterol in Pule'an Tablets by UV spectrophotometry. Methods The determination was performed by using β-sitosterol as control substance. Through the vanillin-high chlorine acid color reaction, the content of total sterols in Pule'an Tablets were determined. Results The absorption wavelength of reference substance and sample maximum were both in the 542 nm. The absorbency and quality of β-sitosterol have good linearity in the range of 0.030 39-0.081 04 mg (r = 0.998 8). The average recovery rate was 100.6% with RSD = 2.75%. Conclusion The method is simple, highly accurate, reproducible, and can be applied to the determination of total sterol in Pule'an Tablets.
[Key words] UV Spectrophotometry; β-sitosterol; Pule'an Tablets; Total sterol; Content determination
普乐安片是以油菜蜂花粉为原料制成的片剂，具有补肾固本的功能，主治肾气亏虚所致的腰膝酸软，尿后余沥或不禁，临床用于前列腺增生和慢性前列腺炎的治疗，疗效确切，安全性高。目前研究结果显示[1-5]，植物甾醇可以很大程度地改善良性前列腺增生(BPH)患者泌尿系统症状，对抑制前列腺肥大和改善膀胱收缩均有明显效果 ......
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