β2-AR基因多态性与婴幼儿毛细支气管炎的相关性研究(1)
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[摘要] 目的 探讨β2-肾上腺素能受体(β2-AR)基因与婴幼儿毛细支气管炎之间的关系。 方法 以毛细支气管炎患儿(n = 131)和正常对照儿童(n = 110)全血DNA为模板，采用PCR的方法扩增β2-AR基因，以DNA直接测序法对目的基因多态性进行分析，采用BLAST将测序结果与NCBI基因库进行相似性序列比较。 结果 毛细支气管炎组与对照组β2-AR 16位点基因型分布及等位基因频率比较差异均有统计学意义(P < 0.05)，两组间β2-AR 27位点等位基因分布差异有统计学意义(P < 0.05)，β2-AR 27位点基因型分布差异无统计学意义(P > 0.05)。不同性别儿童β2-AR基因多态性差异无统计学意义(P > 0.05)。 结论 β2-AR基因多态性和与毛细支气管炎存在一定的有相关性。
[关键词] β2肾上腺素能受体基因；毛细支气管炎；基因多态性
[中图分类号] R725.622.1 [文献标识码] A [文章编号] 1673-7210(2012)08(b)-0010-04
Relationship between genetic polymorphisms of β2-adrenergic receptor and infants bronchiolitis
ZHANG Lei1 ZHANG Jihong1 XU Peiru1,2
1.Department of Pediatrics, the First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830054, China; 2.Institute of Evidence-Based Medicine, Xinjiang Uygur Autonomous Region, Urumqi 830054, China
[Abstract] Objective To study the correlation between genetic polymorphisms of β2-adrenergic receptor (β2-AR) and infants bronchiolitis. Methods The total DNA in the infants of bronchiolitis (n = 131) and normal infants (n = 110) were used as templates, β2-AR gene was amplified by PCR, the gene was detected by DNA sequencing technology, the similarity of sequencing results and NCBI gene bank was detected by BLAST. Results There was statistically difference in the frequencies of genotypes and alleles of β2-AR 16 loci between the two groups (P < 0.05). There was no statistically difference in the frequencies of genotypes of β2-AR 27 loci between the two groups (P > 0.05), but there was statistically difference in the frequencies of alleles of β2-AR 27 loci between the two groups. Conclusion There are some correlation between genetic polymorphisms of β2-AR and infants bronchiolitis ......
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