吡咯烷二硫代氨基甲盐酸对人鼻咽癌CNE—2Z细胞增殖及其细胞核抗原表达的影响(1)
[摘要] 目的 研究核转录因子-κB(NF-κB)特异性抑制剂吡咯烷二硫代氨基甲盐酸(PDTC)对人鼻咽癌CNE-2Z细胞生长增殖及其细胞核抗原(PCNA)的影响。 方法 不同浓度的PDTC(25、50、100 μmol/L)作用CNE-2Z细胞不同时间后(24、48、72 h)，四甲基偶氮唑蓝(MTT)比色法检测PDTC对CNE-2Z细胞增殖的抑制效应；不同浓度的PDTC(25、50、100 μmol/L)作用于CNE-2Z细胞48 h后，流式细胞术(FCM)分析细胞周期，免疫组化(SP)法检测不同强度PDTC作用下CNE-2Z细胞增殖细胞核抗原(PCNA)的表达情况。 结果 25、50、100 μmol/L PDTC处理的CNE-2Z细胞增殖活性明显受到抑制(P < 0.05)，并呈时间和剂量依赖；流式细胞术结果表明S期细胞数减少，细胞受阻于G0/G1期。与对照(无PDTC处理)比较，PDTC作用后CNE-2Z细胞PCNA表达降低(P < 0.05)，并呈明显的剂量依赖。 结论 PDTC具有显著抑制人鼻咽癌CNE-2Z细胞生长的作用，是一种有潜力的抑制鼻咽癌细胞增殖的药物。
, 百拇医药
[关键词] 人鼻咽癌CNE-2Z细胞；吡咯烷二硫代氨基甲盐酸；核转录因子-κB；增殖细胞核抗原
[中图分类号] R286.91 [文献标识码] A [文章编号] 1673-7210(2015)09(a)-0011-05
[Abstract] Objective To explore the effects of pyrrolidine dithiocarbamate (PDTC)，a specific inhibitor of NF-κB on the proliferation of human nasopharyngeal carcinoma cell line CNE-2Z and its effect on proliferation cell nuclear antigen (PCNA) expression. Methods CNE-2Z cells were respectively treated with different concentration (25， 50， 100 μmol/L) PDTC and different time (24， 48， 72 h). The inhibition effects of cell proliferation were assayed by four methyl thiazolyl tetrazolium colorimetric(MTT) method. Cell cycle was analyzed by flow cytometry and the expression of proliferating cell nuclear antigen (PCNA) was detected by immunocytochemical method after treating CNE-2Z cells with different concentration PDTC (25， 50， 100 μmol/L) after 48 h. Results The proliferation activity of CNE-2Z cells dealed with 25， 50， 100 μmol/L PDTC was inhibited obviously (P < 0.05)， and it showed a time-dependent and dose-dependent manner. FCM result showed that the cell population reduced in S phase and the cell cycle was arrested in G0/G1 phase. Compared with the control (no PDTC)， the expression of PCNA of CNE-2Z cells was down regulated after PDTC dealed with (P < 0.05)， and it showed a dose-dependent manner. Conclusion The growth of CNE-2Z can be inhibited and the expression of PCNA in CNE-2Z can could be down-regulated by PDTC. PDTC may be a potential chemotherapeutics for the proliferation of nasopharyngeal carcinoma cell line.
, 百拇医药
[Key words] Human nasopharyngeal carcinoma cell line CNE-2Z； Pyrrolidine dithiocarbamate； NF-κB； Proliferation cell nuclear antigen
鼻咽癌是我国南方地区常见的头颈部恶性肿瘤，治疗以放射治疗为主，目前早期鼻咽癌患者3年生存率约为90%，5年生存率为78%左右；但晚期患者生存率仍不乐观，绝大多数患者死于肿瘤的局部复发和远处转移，5年生存率一直徘徊在40%左右[1]。因此在提高放疗水平的同时，积极探讨化学药物治疗尤为重要。核转录因子-κB(nuclear factor-kappa B，NF-κB)在许多生理、病理过程中发挥着基因调控作用，如炎性反应、免疫应答、细胞增殖与凋亡等，肿瘤的发生、侵袭、转移等过程也与NF-κB密切相关，NF-κB是一类极其重要的多功能核转录因子[2]。NF-κB特异性抑制剂近年在肿瘤防治中的作用受到广泛关注，其作用机制可能是通过抑制核转录因子NF-κB的活化，从而间接抑制下游相关基因的表达，达到抑制肿瘤细胞的生长增殖、侵袭与转移。本实验中选用NF-κB的特异性抑制剂吡咯烷二硫代氨基甲盐酸(pyrrolidine dithiocarbamate，PDTC)作为干预因素，作用于人鼻咽癌CNE-2Z细胞，观察PDTC对CNE-2Z细胞生长增殖及PCNA表达的影响。, http://www.100md.com(向银洲等)
, 百拇医药
[关键词] 人鼻咽癌CNE-2Z细胞；吡咯烷二硫代氨基甲盐酸；核转录因子-κB；增殖细胞核抗原
[中图分类号] R286.91 [文献标识码] A [文章编号] 1673-7210(2015)09(a)-0011-05
[Abstract] Objective To explore the effects of pyrrolidine dithiocarbamate (PDTC)，a specific inhibitor of NF-κB on the proliferation of human nasopharyngeal carcinoma cell line CNE-2Z and its effect on proliferation cell nuclear antigen (PCNA) expression. Methods CNE-2Z cells were respectively treated with different concentration (25， 50， 100 μmol/L) PDTC and different time (24， 48， 72 h). The inhibition effects of cell proliferation were assayed by four methyl thiazolyl tetrazolium colorimetric(MTT) method. Cell cycle was analyzed by flow cytometry and the expression of proliferating cell nuclear antigen (PCNA) was detected by immunocytochemical method after treating CNE-2Z cells with different concentration PDTC (25， 50， 100 μmol/L) after 48 h. Results The proliferation activity of CNE-2Z cells dealed with 25， 50， 100 μmol/L PDTC was inhibited obviously (P < 0.05)， and it showed a time-dependent and dose-dependent manner. FCM result showed that the cell population reduced in S phase and the cell cycle was arrested in G0/G1 phase. Compared with the control (no PDTC)， the expression of PCNA of CNE-2Z cells was down regulated after PDTC dealed with (P < 0.05)， and it showed a dose-dependent manner. Conclusion The growth of CNE-2Z can be inhibited and the expression of PCNA in CNE-2Z can could be down-regulated by PDTC. PDTC may be a potential chemotherapeutics for the proliferation of nasopharyngeal carcinoma cell line.
, 百拇医药
[Key words] Human nasopharyngeal carcinoma cell line CNE-2Z； Pyrrolidine dithiocarbamate； NF-κB； Proliferation cell nuclear antigen
鼻咽癌是我国南方地区常见的头颈部恶性肿瘤，治疗以放射治疗为主，目前早期鼻咽癌患者3年生存率约为90%，5年生存率为78%左右；但晚期患者生存率仍不乐观，绝大多数患者死于肿瘤的局部复发和远处转移，5年生存率一直徘徊在40%左右[1]。因此在提高放疗水平的同时，积极探讨化学药物治疗尤为重要。核转录因子-κB(nuclear factor-kappa B，NF-κB)在许多生理、病理过程中发挥着基因调控作用，如炎性反应、免疫应答、细胞增殖与凋亡等，肿瘤的发生、侵袭、转移等过程也与NF-κB密切相关，NF-κB是一类极其重要的多功能核转录因子[2]。NF-κB特异性抑制剂近年在肿瘤防治中的作用受到广泛关注，其作用机制可能是通过抑制核转录因子NF-κB的活化，从而间接抑制下游相关基因的表达，达到抑制肿瘤细胞的生长增殖、侵袭与转移。本实验中选用NF-κB的特异性抑制剂吡咯烷二硫代氨基甲盐酸(pyrrolidine dithiocarbamate，PDTC)作为干预因素，作用于人鼻咽癌CNE-2Z细胞，观察PDTC对CNE-2Z细胞生长增殖及PCNA表达的影响。, http://www.100md.com(向银洲等)