miR—21表达量与动脉粥样硬化程度的关系及长春西汀对其影响(1)
[摘要] 目的 研究不同程度动脉粥样硬化与miR-21表达量的相关性，以及应用长春西汀后对miR-21表达量的影响。 方法 选取2014年1月～2016年12月于首都医科大学附属北京友谊医院医疗保健中心住院的动脉粥样硬化患者40例，按动脉粥样硬化程度将其分为轻度(9例)、中度(6例)和重度(25例)，按治疗方法将其分为用药组(17例)和对照组(23例)。提取患者的血清RNA并利用实时定量PCR方法分析不同程度动脉粥样硬化患者血清中miR-21水平，比较用药组和对照组治疗前后miR-21含量和血细胞比容的变化。 结果 重度动脉粥样硬化患者血清miR-21表达水平明显高于轻度动脉粥样硬化患者，差异有统计学意义(P < 0.05)。治疗后，用药组血细胞比容、miR-21相对含量均较治疗前明显下降，差异有统计学意义(P < 0.05)，用药组和对照组治疗后miR-21相对含量比较，差异无统计学意义(P > 0.05)。 结论 动脉粥样硬化患者血清miR-21水平随动脉粥样硬化程度的增高而增高，经长春西汀治疗后，miR-21表达水平明显下调，提示miR-21与动脉粥样硬化程度相关。
[关键词] 长春西汀；动脉粥样硬化；微小RNA
[中图分类号] R541.4 [文献标识码] A [文章编号] 1673-7210(2017)06(c)-0161-04
[Abstract] Objective To study the correlation between different degrees of atherosclerosis and the expression quantity of miR-21， and the influence of Vinpocetine application on the expression quantity of miR-21. Methods Forty patients with atherosclerosis hospitalized in Medical Care Center， Beijing Friendship Hospital， Capital Medical University from January 2014 to December 2016 were selected， and they were divided into mild (9 cases)， moderate (6 cases) and severe (25 cases) according to the degree of atherosclerosis， and they were also divided into medication group (17 cases) and control group (23 cases) according to the treatment method. The serum RNA of patients was extracted and the levels of serum miR-21 in the patients with different degrees of atherosclerosis were analyzed by real-time quantitative PCR， the changes of miR-21 and haematocrit before and after treatment in the medication group and control group were compared. Results The expression of miR-21 in patients with severe atherosclerosis was higher than that in patients with mild atherosclerosis， the difference was statistically significant (P < 0.05). After treatment， the haematocrit and relative contents of miR-21 in the medication group were lower than those before treatment， the differences were statistically significant (P < 0.05)， there was no statistically significant difference of the relative contents of miR-21 after treatment between medication group and control group (P > 0.05). Conclusion The levels of serum miR-21 in patients with atherosclerosis are increased with the increase of atherosclerosis， and the expression of miR-21 is significantly decreased after treatment with Vinpocetine， which indicates that miR-21 has correlation with atherosclerosis.
[Key words] Vincristine； Atherosclerosis； MicroRNA
動脉粥样硬化以动脉壁的脂质沉积、功能紊乱及斑块形成、慢性炎症细胞浸润为主要特征，是公认的一种慢性炎症疾病[1]，其预防与治疗是目前心脑血管专家关注的焦点之一[2]。miR-21是一类非编码RNA，其作为原癌基因在多种肿瘤中高表达，并参与细胞的增生、分化、凋亡等过程，在肿瘤的发生发展中具有重要作用[3]。而近期有研究表明，miR-21在脑梗死、冠心病等动脉粥样硬化相关性疾病中表达明显增高[4-5]。长春西汀具有抑制血小板聚集、降低血液黏度、改善血液微循环和流动性等作用[6-8]，且可改善大动脉粥样硬化。本研究着眼于明确动脉粥样硬化程度与miR-21含量之间的关系，以及应用长春西汀后对miR-21表达量的影响，为研究miR-21表达水平对动脉粥样硬化程度的评估和发生发展机制研究提供理论支持。, http://www.100md.com(王珊 杨华昱 姚兰 汪云超 陈海平 )
[关键词] 长春西汀；动脉粥样硬化；微小RNA
[中图分类号] R541.4 [文献标识码] A [文章编号] 1673-7210(2017)06(c)-0161-04
[Abstract] Objective To study the correlation between different degrees of atherosclerosis and the expression quantity of miR-21， and the influence of Vinpocetine application on the expression quantity of miR-21. Methods Forty patients with atherosclerosis hospitalized in Medical Care Center， Beijing Friendship Hospital， Capital Medical University from January 2014 to December 2016 were selected， and they were divided into mild (9 cases)， moderate (6 cases) and severe (25 cases) according to the degree of atherosclerosis， and they were also divided into medication group (17 cases) and control group (23 cases) according to the treatment method. The serum RNA of patients was extracted and the levels of serum miR-21 in the patients with different degrees of atherosclerosis were analyzed by real-time quantitative PCR， the changes of miR-21 and haematocrit before and after treatment in the medication group and control group were compared. Results The expression of miR-21 in patients with severe atherosclerosis was higher than that in patients with mild atherosclerosis， the difference was statistically significant (P < 0.05). After treatment， the haematocrit and relative contents of miR-21 in the medication group were lower than those before treatment， the differences were statistically significant (P < 0.05)， there was no statistically significant difference of the relative contents of miR-21 after treatment between medication group and control group (P > 0.05). Conclusion The levels of serum miR-21 in patients with atherosclerosis are increased with the increase of atherosclerosis， and the expression of miR-21 is significantly decreased after treatment with Vinpocetine， which indicates that miR-21 has correlation with atherosclerosis.
[Key words] Vincristine； Atherosclerosis； MicroRNA
動脉粥样硬化以动脉壁的脂质沉积、功能紊乱及斑块形成、慢性炎症细胞浸润为主要特征，是公认的一种慢性炎症疾病[1]，其预防与治疗是目前心脑血管专家关注的焦点之一[2]。miR-21是一类非编码RNA，其作为原癌基因在多种肿瘤中高表达，并参与细胞的增生、分化、凋亡等过程，在肿瘤的发生发展中具有重要作用[3]。而近期有研究表明，miR-21在脑梗死、冠心病等动脉粥样硬化相关性疾病中表达明显增高[4-5]。长春西汀具有抑制血小板聚集、降低血液黏度、改善血液微循环和流动性等作用[6-8]，且可改善大动脉粥样硬化。本研究着眼于明确动脉粥样硬化程度与miR-21含量之间的关系，以及应用长春西汀后对miR-21表达量的影响，为研究miR-21表达水平对动脉粥样硬化程度的评估和发生发展机制研究提供理论支持。, http://www.100md.com(王珊 杨华昱 姚兰 汪云超 陈海平 )