柚皮素对人肺鳞癌NCI—H2170细胞增殖、凋亡的影响(1)
[摘要] 目的 探討柚皮素对人肺鳞癌细胞NCI-H2170的增殖、凋亡及细胞周期的影响。 方法 用MTT法检测柚皮素对NCI-H2170细胞增殖的影响，Hoechst33258/PI染色检测NCI-H2170细胞形态学变化，流式细胞术(FCM)检测细胞周期的改变。Western blot检测柚皮素处理NCI-H2170细胞24 h后PARP、Bcl-2、Bid、procaspase-3及procaspase-8蛋白的表达。 结果 柚皮素处理人肺鳞癌NCI-H2170细胞24 h后，浓度依赖性地抑制NCI-H2170细胞增殖；其中12.5 μmol/L处理组与对照组比较，差异无统计学(P > 0.05)；25、50、100 μmol/L处理组与对照组比较，差异有统计学意义(P < 0.05)。流式细胞仪分析结果显示：不同浓度的柚皮素处理NCI-H2170细胞24 h后，与对照组比较，12.5 μmol/L处理组差异无统计学意义(P > 0.05)；25、50、100 μmol/L处理组G1期细胞显著增多，S期及G2期细胞显著减少，差异均有统计学意义(P < 0.05)。25、50、100 μmol/L处理组与对照组相比，柚皮素上调Bid表达，促进PARP降解，Bcl-2、procaspase-3和procaspase-8降低，差异均有统计学意义(P < 0.05)。 结论 柚皮素抑制人肺鳞癌NCI-H2170细胞增殖，并能诱导NCI-H2170细胞产生G1期阻滞，诱导细胞凋亡。
[关键词] 柚皮素；人肺鳞癌NCI-H2170细胞；细胞凋亡；增殖；细胞周期
[中图分类号] R734.2 [文献标识码] A [文章编号] 1673-7210(2018)03(b)-0004-05
Effects of Naringenin on the proliferation and apoptosis of lung cancer cell NCI-H2170
ZENG Degui1* CHEN Bao′an2* WU Shang3 HUANG Hui3 WU jun1，2
1.Department of Pharmacy， Shenzhen Center for Chronic Disease Prevention and Control， Guangdong Province， Shenzhen 518020， China； 2.Institute of Respiratory Diseases， Guangdong Medical University， Guangdong Province， Zhanjiang 524023， China； 3.Department of Biochemistry and Molecular Biology， Guangdong Medical University， Zhanjiang ， Guangdong 524023， China
[Abstract] Objective To investigate the effects of Naringenin on the proliferation， apoptosis and cell cycle of human lung squamous cell carcinoma cell line NCI-H2170. Methods The proliferation of NCI-H2170 cells was detected by MTT assay. The morphological changes of NCI-H2170 cells were detected by Hoechst33258/PI staining. Cell cycle changes were measured by flow cytometry (FCM). Western blot was used to detect the expression of PARP， Bid， Bcl-2， procaspase-3 and procaspase-8 in NCI-H2170 cells treated with naringenin. Results The proliferation of human lung squamous cell carcinoma NCI-H2170 cells were inhibited in a dose-dependent manner after treating with Naringenin for 24 h. It was not significant difference between 12.5 μmol/L treatment group and the control group (P > 0.05).However， 25， 50， 100 μmol/L treatment groups were significantly different comparing to the control group (P < 0.05). The results of flow cytometry analysis showed that it was not significant difference between 12.5 μmol/L treatment group and the control group (P > 0.05). However， after NCI-H2170 cells were treated with 25， 50， 100 μmol/L naringenins， the number of cells had significantly increased in G1 phase， desceased in S phase and G2 phase， comparing to the control group (P < 0.05). Naringenin up-regulated Bid expression， promoted the degradation of PARP and the decrease of Bcl-2， proaspase-3 and proaspase-8 in NCI-H2170 cells， comparing to the control group (P < 0.05). Conclusion Naringenin inhibits the proliferation of human lung squamous cell carcinoma cell line NCI-H2170 and induces the G1 phase arrest in NCI-H2170 cells and induces cell apoptosis., 百拇医药(曾德贵 陈保安 吴尚 黄慧 伍俊)
[关键词] 柚皮素；人肺鳞癌NCI-H2170细胞；细胞凋亡；增殖；细胞周期
[中图分类号] R734.2 [文献标识码] A [文章编号] 1673-7210(2018)03(b)-0004-05
Effects of Naringenin on the proliferation and apoptosis of lung cancer cell NCI-H2170
ZENG Degui1* CHEN Bao′an2* WU Shang3 HUANG Hui3 WU jun1，2
1.Department of Pharmacy， Shenzhen Center for Chronic Disease Prevention and Control， Guangdong Province， Shenzhen 518020， China； 2.Institute of Respiratory Diseases， Guangdong Medical University， Guangdong Province， Zhanjiang 524023， China； 3.Department of Biochemistry and Molecular Biology， Guangdong Medical University， Zhanjiang ， Guangdong 524023， China
[Abstract] Objective To investigate the effects of Naringenin on the proliferation， apoptosis and cell cycle of human lung squamous cell carcinoma cell line NCI-H2170. Methods The proliferation of NCI-H2170 cells was detected by MTT assay. The morphological changes of NCI-H2170 cells were detected by Hoechst33258/PI staining. Cell cycle changes were measured by flow cytometry (FCM). Western blot was used to detect the expression of PARP， Bid， Bcl-2， procaspase-3 and procaspase-8 in NCI-H2170 cells treated with naringenin. Results The proliferation of human lung squamous cell carcinoma NCI-H2170 cells were inhibited in a dose-dependent manner after treating with Naringenin for 24 h. It was not significant difference between 12.5 μmol/L treatment group and the control group (P > 0.05).However， 25， 50， 100 μmol/L treatment groups were significantly different comparing to the control group (P < 0.05). The results of flow cytometry analysis showed that it was not significant difference between 12.5 μmol/L treatment group and the control group (P > 0.05). However， after NCI-H2170 cells were treated with 25， 50， 100 μmol/L naringenins， the number of cells had significantly increased in G1 phase， desceased in S phase and G2 phase， comparing to the control group (P < 0.05). Naringenin up-regulated Bid expression， promoted the degradation of PARP and the decrease of Bcl-2， proaspase-3 and proaspase-8 in NCI-H2170 cells， comparing to the control group (P < 0.05). Conclusion Naringenin inhibits the proliferation of human lung squamous cell carcinoma cell line NCI-H2170 and induces the G1 phase arrest in NCI-H2170 cells and induces cell apoptosis., 百拇医药(曾德贵 陈保安 吴尚 黄慧 伍俊)