御唐丸对糖尿病大鼠胰腺组织IRS—1基因表达和蛋白磷酸化的影响(1)
[摘要] 目的 探討御唐丸对糖尿病大鼠胰腺组织胰岛素受体底物-1(IRS-1)基因表达和蛋白磷酸化的影响。 方法 将150只SD大鼠按体重分层随机分为空白组(20只)及造模组(130只)。用高脂饲料连续喂养造模组大鼠10周制备2型糖尿病大鼠模型，按随机数字表法分为模型组、糖尿乐组、御唐丸高剂量组、御唐丸低剂量组及格华止组，每组20只。按大鼠与成人等效剂量折算，御唐丸高剂量组给予御唐丸2.70 g/(kg·d)，御唐丸低剂量组给予御唐丸1.35 g/(kg·d)，糖尿乐组给予糖尿乐2.43 g/(kg·d)，格华止组给予格华止0.09 g/(kg·d)，模型组及空白组给予生理盐水2 mL/d。连续灌胃给药8周后取大鼠胰腺组织，用Real-time PCR方法检测IRS-1 mRNA表达水平，用Western blot法检测IRS-1蛋白表达及磷酸化水平。 结果 与模型组比较，格华止组、糖尿乐组、御唐丸低剂量组及御唐丸高剂量组IRS-1 mRNA的相对表达量均有所增加(P < 0.05或P < 0.01)，且御唐丸高剂量组IRS-1 mRNA的相对表达量高于御唐丸低剂量组(P < 0.05)；与模型组比较，格华止组、糖尿乐组、御唐丸低剂量组及御唐丸高剂量组IRS-1 ser307蛋白磷酸化水平均显著降低(P < 0.01)，且御唐丸高剂量组IRS-1 ser307蛋白磷酸化水平显著低于御唐丸低剂量组，差异有高度统计学意义(P < 0.01)。 结论 御唐丸能通过影响IRS-1 mRNA相对表达量、抑制IRS-1 ser307蛋白磷酸化进程从而减轻胰岛素抵抗，进而对2型糖尿病起到治疗作用。
[关键词] 2型糖尿病；健脾益肾；养气补阴；胰岛素受体底物-1
[中图分类号] R587.1 [文献标识码] A [文章编号] 1673-7210(2018)10(c)-0011-05
[Abstract] Objective To study the effects of Yutang Pills on gene expression of insulin receptor substrate-1 (IRS-1) and protein phosphorylation in pancreatic tissue of diabetic rats. Methods In this study， 150 SD rats were randomly divided into two groups according to their weight， with 20 rats in the blank group and 130 rats in the model group. The model group was fed with high fat forage for 10 weeks to prepare type 2 diabetic rat models and then divided into model control group， Tangniaole group， high dosage of Yutang Pills group， low dosage of Yutang Pills group and Glucophage group according to random number table method， with 20 rats in each group. With the equivalent dosage of rats and adults， the high dosage of Yutang Pills group was given 2.70 g/(kg·d) of Yutang Pills， low dosage of Yutang Pills group was given 1.35 g/(kg·d) of Yutang Pills， Tangniaole group was given 2.43 g/(kg·d) of Tangniaole， and Glucophage group was given 0.09 g/(kg·d) of Glucophage， meanwhile， the model control group and the blank group were given 2 mL/d of normal saline. The pancreatic tissues of rats were taken after 8 weeks of continuous intragastric administration， IRS-1 mRNA expression level was detected by Real-time PCR， and IRS-1 protein expression and phosphorylation levels were detected by Western blot. Results Compared with the model control group， the relative expression of IRS-1 mRNA in Glucophage group， Tangniaole group， low dosage of Yutang Pills group and high dosage of Yutang Pills group was increased (P < 0.05 or P < 0.01)， and the relative expression of IRS-1 mRNA in high dosage of Yutang Pills group was higher than that in low dosage of Yutang Pills group (P < 0.05). Compared with the model control group， the levels of IRS-1 ser307 protein phosphorylation in Glucophage group， Tangniaole group， low dosage of Yutang Pills group and high dosage of Yutang Pills group were significantly decreased (P < 0.01)， and the level of IRS-1 ser307 protein phosphorylation in high dosage of Yutang Pills group was lower than that in low dosage of Yutang Pills group， the difference was highly statistically significant (P < 0.01). Conclusion Yutang Pills can play a therapeutic role in type 2 diabetes mellitus by affecting the relative expression of IRS-1 mRNA and inhibiting the progress of ser307 protein phosphorylation on IRS-1 to reduce insulin resistance., http://www.100md.com(郑南 谢宁 张佩青 高丽娟)
[关键词] 2型糖尿病；健脾益肾；养气补阴；胰岛素受体底物-1
[中图分类号] R587.1 [文献标识码] A [文章编号] 1673-7210(2018)10(c)-0011-05
[Abstract] Objective To study the effects of Yutang Pills on gene expression of insulin receptor substrate-1 (IRS-1) and protein phosphorylation in pancreatic tissue of diabetic rats. Methods In this study， 150 SD rats were randomly divided into two groups according to their weight， with 20 rats in the blank group and 130 rats in the model group. The model group was fed with high fat forage for 10 weeks to prepare type 2 diabetic rat models and then divided into model control group， Tangniaole group， high dosage of Yutang Pills group， low dosage of Yutang Pills group and Glucophage group according to random number table method， with 20 rats in each group. With the equivalent dosage of rats and adults， the high dosage of Yutang Pills group was given 2.70 g/(kg·d) of Yutang Pills， low dosage of Yutang Pills group was given 1.35 g/(kg·d) of Yutang Pills， Tangniaole group was given 2.43 g/(kg·d) of Tangniaole， and Glucophage group was given 0.09 g/(kg·d) of Glucophage， meanwhile， the model control group and the blank group were given 2 mL/d of normal saline. The pancreatic tissues of rats were taken after 8 weeks of continuous intragastric administration， IRS-1 mRNA expression level was detected by Real-time PCR， and IRS-1 protein expression and phosphorylation levels were detected by Western blot. Results Compared with the model control group， the relative expression of IRS-1 mRNA in Glucophage group， Tangniaole group， low dosage of Yutang Pills group and high dosage of Yutang Pills group was increased (P < 0.05 or P < 0.01)， and the relative expression of IRS-1 mRNA in high dosage of Yutang Pills group was higher than that in low dosage of Yutang Pills group (P < 0.05). Compared with the model control group， the levels of IRS-1 ser307 protein phosphorylation in Glucophage group， Tangniaole group， low dosage of Yutang Pills group and high dosage of Yutang Pills group were significantly decreased (P < 0.01)， and the level of IRS-1 ser307 protein phosphorylation in high dosage of Yutang Pills group was lower than that in low dosage of Yutang Pills group， the difference was highly statistically significant (P < 0.01). Conclusion Yutang Pills can play a therapeutic role in type 2 diabetes mellitus by affecting the relative expression of IRS-1 mRNA and inhibiting the progress of ser307 protein phosphorylation on IRS-1 to reduce insulin resistance., http://www.100md.com(郑南 谢宁 张佩青 高丽娟)