急性期川崎病患儿血浆TSP-1、PAF水平变化及临床意义(1)
[摘要] 目的 研究急性期川崎病(KD)患兒血浆血小板活化因子(PAF)和血小板反应蛋白-1(TSP-1)水平的变化及临床意义。 方法 选取2015年1月～2018年1月四川大学华西临床医学院(以下简称“我院”)诊治的107例KD患儿为KD组，根据超声心动图表现将其分为冠脉扩张组(n = 43)与无冠脉扩张组(n = 64);选取我院同期30例拟行腹股沟斜疝的患儿作为对照组。采用酶联免疫吸附(ELISA)法检测KD组急性期、亚急性期、恢复期及对照组血浆TSP-1、PAF、血小板计数(PLT)含量。比较各组不同指标间的差异。 结果 KD组患儿急性期血浆PAF、TSP-1及PLT明显高于对照组(均P < 0.05)，KD组血浆PAF、TSP-1水平恢复期、亚急性期、急性期呈升高趋势(均P < 0.05)。冠脉扩张组患儿急性期血浆PAF水平明显高于无冠脉扩张组(P < 0.05)，而亚急性期及恢复期血浆PAF水平与无冠脉扩张组比较，差异无统计学意义(均P > 0.05)。冠脉扩张组患儿急性期、亚急性期及恢复期TSP-1水平及PLT与无冠脉扩张组比较，差异无统计学意义(P > 0.05)。KD组患儿急性期血浆TSP-1与PAF、PLT不相关(r = 0.314、0.051，P > 0.05)，而PAF与PLT呈显著正相关(r = 0.614，P < 0.05)。 结论 KD急性期血浆PAF、TSP-1明显升高，PAF在冠脉损伤时升高明显，二者参与KD的发生发展，有可能成为早期诊断KD的标志物。
[关键词] 川崎病;急性期;血小板活化因子;血小板反应蛋白-1
[中图分类号] R725.4 [文献标识码] A [文章编号] 1673-7210(2019)07(a)-0113-04
Changes of plasma TSP-1 and PAF level in children with acute Kawasaki disease and their clinical significance
JIANG Yongmei1，2 CHEN Tingting3 ZHOU Kaiyu4 LAI Meimei5
1.West China Hospital of Sichuan University， Sichuan Province， Chengdu 610041， China; 2.Infectious Disease Department， Chengdu Women′s and Children′s Central Hospital， Sichuan Province， Chengdu 610017， China; 3.Department of Cardiology， Chengdu Women′s and Children′s Central Hospital， Sichuan Province， Chengdu 610017， China; 4.Department of Cardiology， West China Women′s and Children′s Hospital， Sichuan Province， Chengdu 610041， China; 5.Department of Laboratory， Chengdu Women′s and Children′s Central Hospital， Sichuan Province， Chengdu 610017， China
[Abstract] Objective To study the changes and clinical significance of plasma platelet activating factor (PAF) and thrombospondin-1 (TSP-1) levels in children with acute Kawasaki disease (KD). Methods A total of 107 children with KD who were treated in West China Hospital of Sichuan University ("our hospital" for short) from January 2015 to January 2018 were selected as KD group. They were divided into coronary artery expansion group (n = 43) and non-coronary expansion group (n = 64) according to echocardiographic findings. Thirty cases of indirect inguinal hernia in our hospital in the same period were selected as control group. The levels of TSP-1， PAF and platelets(PLT) in the acute， subacute and recovery phases of the KD group and the control group were detected by enzyme-linked immunosorbent assay (ELISA). The differences among different indicators of each group were compared. Results The levels of plasma PAF， TSP-1 and PLT in KD group with the acute phase were significantly higher than those in control group (all P < 0.05). The plasma levels of PAF and TSP-1 in KD group increased in recovery， subacute and acute stages (all P < 0.05). The plasma PAF level in coronary artery injury group was significantly higher than that in non-coronary injury group (P < 0.05)， but there was no significant difference in plasma PAF level between KD group in the subacute and recovery phases and non-coronary injury group (P > 0.05). There was no significant differences in TSP-1 level and PLT count between coronary artery injury group in the acute， subacute， and recovery phase and non-coronary injury group (P > 0.05). There was no correlation between plasma TSP-1 and PAF， PLT in KD group in the acute phase(r = 0.314， 0.051， P > 0.05)， but there was a significant positive correlation between PAF and PLT (r = 0.614， P < 0.05). Conclusion The plasma levels of PAF and TSP-1 are significantly increased in the acute phase of KD， and PAF is significantly increased in coronary artery injury. Both PAF and TSP-1 are involved in the occurrence and development of KD， and might be used as markers for early diagnosis of KD., http://www.100md.com(蒋永梅 陈婷婷 周开宇 赖梅梅)
[关键词] 川崎病;急性期;血小板活化因子;血小板反应蛋白-1
[中图分类号] R725.4 [文献标识码] A [文章编号] 1673-7210(2019)07(a)-0113-04
Changes of plasma TSP-1 and PAF level in children with acute Kawasaki disease and their clinical significance
JIANG Yongmei1，2 CHEN Tingting3 ZHOU Kaiyu4 LAI Meimei5
1.West China Hospital of Sichuan University， Sichuan Province， Chengdu 610041， China; 2.Infectious Disease Department， Chengdu Women′s and Children′s Central Hospital， Sichuan Province， Chengdu 610017， China; 3.Department of Cardiology， Chengdu Women′s and Children′s Central Hospital， Sichuan Province， Chengdu 610017， China; 4.Department of Cardiology， West China Women′s and Children′s Hospital， Sichuan Province， Chengdu 610041， China; 5.Department of Laboratory， Chengdu Women′s and Children′s Central Hospital， Sichuan Province， Chengdu 610017， China
[Abstract] Objective To study the changes and clinical significance of plasma platelet activating factor (PAF) and thrombospondin-1 (TSP-1) levels in children with acute Kawasaki disease (KD). Methods A total of 107 children with KD who were treated in West China Hospital of Sichuan University ("our hospital" for short) from January 2015 to January 2018 were selected as KD group. They were divided into coronary artery expansion group (n = 43) and non-coronary expansion group (n = 64) according to echocardiographic findings. Thirty cases of indirect inguinal hernia in our hospital in the same period were selected as control group. The levels of TSP-1， PAF and platelets(PLT) in the acute， subacute and recovery phases of the KD group and the control group were detected by enzyme-linked immunosorbent assay (ELISA). The differences among different indicators of each group were compared. Results The levels of plasma PAF， TSP-1 and PLT in KD group with the acute phase were significantly higher than those in control group (all P < 0.05). The plasma levels of PAF and TSP-1 in KD group increased in recovery， subacute and acute stages (all P < 0.05). The plasma PAF level in coronary artery injury group was significantly higher than that in non-coronary injury group (P < 0.05)， but there was no significant difference in plasma PAF level between KD group in the subacute and recovery phases and non-coronary injury group (P > 0.05). There was no significant differences in TSP-1 level and PLT count between coronary artery injury group in the acute， subacute， and recovery phase and non-coronary injury group (P > 0.05). There was no correlation between plasma TSP-1 and PAF， PLT in KD group in the acute phase(r = 0.314， 0.051， P > 0.05)， but there was a significant positive correlation between PAF and PLT (r = 0.614， P < 0.05). Conclusion The plasma levels of PAF and TSP-1 are significantly increased in the acute phase of KD， and PAF is significantly increased in coronary artery injury. Both PAF and TSP-1 are involved in the occurrence and development of KD， and might be used as markers for early diagnosis of KD., http://www.100md.com(蒋永梅 陈婷婷 周开宇 赖梅梅)