白细胞分化抗原147、血管内皮生长因子蛋白表达与肺腺癌患者临床分期、淋巴结转移的关系(1)
[摘要] 目的 探讨白细胞分化抗原147(CD147)、血管内皮生长因子(VEGF)蛋白表达与肺腺癌患者的临床分期、淋巴结转移的关系。 方法 选取昆明医科大学第三附属医院2015年10月～2018年10月肺腺癌患者78例，取其肺腺癌组织与相应癌旁正常肺组织标本，以免疫组织化学SP法检测患者肺腺癌组织和相应癌旁正常肺组织标本中CD147、VEGF蛋白表达，并分析CD147、VEGF蛋白表达和患者淋巴结转移、临床分期之间关系。 结果 肺腺癌组织中CD147、VEGF蛋白阳性表达率均高于癌旁正常肺组织，差异有统计学意义(P < 0.05);有淋巴结转移、临床分期Ⅲ～Ⅳ期患者的肺腺癌组织中CD147、VEGF蛋白阳性表达率均高于无淋巴结转移及临床分期Ⅰ～Ⅱ期患者，差异均有统计学意义(均P < 0.05)。 结论 肺腺癌组织中CD147、VEGF蛋白均呈高表达，且两者表达水平与淋巴结转移、临床分期相关，可将其作为肺腺癌诊断、恶性程度评估的重要指标。
[关键词] 肺腺癌;白细胞分化抗原147;血管内皮生长因子;临床分期;淋巴结转移
[中图分类号] R736.4 [文献标识码] A [文章编号] 1673-7210(2019)08(b)-0127-04
[Abstract] Objective To investigate the relationship between the expression of leukocyte differentiation antigen 147 (CD147) and vascular endothelial growth factor (VEGF) protein and clinical stage， lymph node metastasis in patients with lung adenocarcinoma. Methods A total of 78 patients with lung adenocarcinoma from October 2015 to October 2018 in the Third Affiliated Hospital of Kunming Medical University were enrolled. The lung adenocarcinoma tissues and corresponding normal lung tissues were taken and the lung glands were detected by immunohistochemical SP method. The expressions of CD147 and VEGF protein in cancer tissues and corresponding normal lung tissues were analyzed， and the relationship between CD147， VEGF protein expression and lymph node metastasis and clinical stage was analyzed. Results The positive expression rates of CD147 and VEGF protein in lung adenocarcinoma tissues were higher than those in corresponding normal lung tissues， the differences were statistically significant (P < 0.05). The positive expression rates of CD147 and VEGF protein in lung adenocarcinoma tissues with lymph node metastasis and clinical stage Ⅲ-Ⅳ were higher than those in non-lymph node metastasis and clinical stage Ⅰ-Ⅱ patients， the differences were statistically significant (all P < 0.05). Conclusion The expressions of CD147 and VEGF in lung adenocarcinoma are highly expressed， and their expression levels are related to lymph node metastasis and clinical stage. They can be used as an important index for the diagnosis and malignant degree evaluation of lung adenocarcinoma.
[Key words] Lung adenocarcinoma; Leukocyte differentiation antigen 147; Vascular endothelial growth factor; Clinical stage; Lymph node metastasis
近年来，随大气污染情况与人口老龄化加剧、主动与被动吸烟人数增加等因素影响，肺癌发病率逐渐增高，每年肺癌发病人数占据全部癌症发病总人数的13%左右，且病死人数约占全部癌症病死总人数的18%，居各类型恶性肿瘤之首[1-3]。临床研究[4-5]报道，肺癌起病较隐匿，75%左右肺癌患者于疾病确诊时已进展中晚期，存在转移证据，致使治疗难度及患者病死率提升。故探寻一种肺癌早期诊断及病情评价的灵敏方法对指导临床采取及时准确治疗、延长患者生存周期均有重要意义。白细胞分化抗原147(CD147)为免疫球蛋白超家族中的一员，可刺激肿瘤附近间质纤维母细胞分泌大量基质金属蛋白酶，参与细胞外基质降解，与肿瘤转移侵袭密切相关[6]。血管内皮生长因子(VEGF)为一種正性血管生成调节因子，能促进肿瘤及组织新生血管生成，在肿瘤发生、进展、侵袭中起着重要作用[7-8]。此外，肺腺癌为肺癌重要类型之一，其在肺癌中占据着较大比例。本研究以免疫组织化学SP法测定患者肺腺癌组织和相应癌旁正常肺组织标本中CD147、VEGF蛋白表达，旨在分析其表达和患者临床分期、淋巴结转移等病理参数之间关系，为临床早期诊断、病情评估和靶向治疗提供参考。, http://www.100md.com(杨继岚 胡凤娣 谢琳)
[关键词] 肺腺癌;白细胞分化抗原147;血管内皮生长因子;临床分期;淋巴结转移
[中图分类号] R736.4 [文献标识码] A [文章编号] 1673-7210(2019)08(b)-0127-04
[Abstract] Objective To investigate the relationship between the expression of leukocyte differentiation antigen 147 (CD147) and vascular endothelial growth factor (VEGF) protein and clinical stage， lymph node metastasis in patients with lung adenocarcinoma. Methods A total of 78 patients with lung adenocarcinoma from October 2015 to October 2018 in the Third Affiliated Hospital of Kunming Medical University were enrolled. The lung adenocarcinoma tissues and corresponding normal lung tissues were taken and the lung glands were detected by immunohistochemical SP method. The expressions of CD147 and VEGF protein in cancer tissues and corresponding normal lung tissues were analyzed， and the relationship between CD147， VEGF protein expression and lymph node metastasis and clinical stage was analyzed. Results The positive expression rates of CD147 and VEGF protein in lung adenocarcinoma tissues were higher than those in corresponding normal lung tissues， the differences were statistically significant (P < 0.05). The positive expression rates of CD147 and VEGF protein in lung adenocarcinoma tissues with lymph node metastasis and clinical stage Ⅲ-Ⅳ were higher than those in non-lymph node metastasis and clinical stage Ⅰ-Ⅱ patients， the differences were statistically significant (all P < 0.05). Conclusion The expressions of CD147 and VEGF in lung adenocarcinoma are highly expressed， and their expression levels are related to lymph node metastasis and clinical stage. They can be used as an important index for the diagnosis and malignant degree evaluation of lung adenocarcinoma.
[Key words] Lung adenocarcinoma; Leukocyte differentiation antigen 147; Vascular endothelial growth factor; Clinical stage; Lymph node metastasis
近年来，随大气污染情况与人口老龄化加剧、主动与被动吸烟人数增加等因素影响，肺癌发病率逐渐增高，每年肺癌发病人数占据全部癌症发病总人数的13%左右，且病死人数约占全部癌症病死总人数的18%，居各类型恶性肿瘤之首[1-3]。临床研究[4-5]报道，肺癌起病较隐匿，75%左右肺癌患者于疾病确诊时已进展中晚期，存在转移证据，致使治疗难度及患者病死率提升。故探寻一种肺癌早期诊断及病情评价的灵敏方法对指导临床采取及时准确治疗、延长患者生存周期均有重要意义。白细胞分化抗原147(CD147)为免疫球蛋白超家族中的一员，可刺激肿瘤附近间质纤维母细胞分泌大量基质金属蛋白酶，参与细胞外基质降解，与肿瘤转移侵袭密切相关[6]。血管内皮生长因子(VEGF)为一種正性血管生成调节因子，能促进肿瘤及组织新生血管生成，在肿瘤发生、进展、侵袭中起着重要作用[7-8]。此外，肺腺癌为肺癌重要类型之一，其在肺癌中占据着较大比例。本研究以免疫组织化学SP法测定患者肺腺癌组织和相应癌旁正常肺组织标本中CD147、VEGF蛋白表达，旨在分析其表达和患者临床分期、淋巴结转移等病理参数之间关系，为临床早期诊断、病情评估和靶向治疗提供参考。, http://www.100md.com(杨继岚 胡凤娣 谢琳)