单分子检测技术及其在人涎腺肿瘤实验性转移中的应用
1全内反射荧光显微术,2分子光学成像在人涎腺肿瘤实验性转移中的应用,3展望,4参考文献,绿色荧光蛋白,实时观测
[摘要]单分子检测技术是对宏观表象本质进行直接探索的一种方法,其检测空间尺度可至纳米数量级,考察异质群体中的每个个体,并鉴定、分类、定量比较它们的亚群。单分子检测技术可以用来检测生物系统中小概率事件或数目较少的目标分子。本文以全内反射荧光显微术为例概述单分子检测技术及其在口腔医学中的运用。[关键词]单分子检测;全内反射荧光显微术;绿色荧光蛋白;实时观测
[中图分类号]R 782[文献标志码]A[doi]10.3969/j.issn.1673-5749.2012.04.026
Single-molecule detection technology and its application of experimental metastasis of human salivary tu-morTian Tian, Li Shu.(Dept. of Periodontics, Stomatological Hospital of Shandong University, Jinan 250012, China; Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China)
[Abstract]Single-molecule detection(SMD)directly explores the essence of macro presentation by detect scale of nanometer scalar which can review every unit of the heterogeneous group and identify, classify, measurable compare its subgroups. SMD can sensitively detect small probability event or minority aim molecules of the biology system. This paper reviews total internal reflection fluorescence and applications in the life sciences.
[Key words]single-molecule detection;total internal reflection fluorescence;green fluorescent protein;realtime observation ......
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